Toxoplasma gondii

What a Difference 30 years Makes! A Perspective on Changes in research Methodologies used to Study Toxoplasma gondii.- ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms.- Isolation and Genotyping of Toxoplasma gondii Strains.- Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of their Sporozoites for Downstream Manipulation.- Assay for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse.- PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plantes without DNA Purification.- CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma gondii.- Assessing Rhyptry Secretion in T. gondii.- High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii.- Plate-Based Quantification of Stimulated Toxoplasma egress.- Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii.- Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging.- Experimental Approaches for Examining Apicoplast Biology.- Measuring Solute Transport in Toxoplasma gondii Parasites.- Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo.- Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ.- Model Systems for Studying Mechanisms of Ocular Toxoplasmosis.- Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii.- Assays to Evaluate Toxoplasma-Macrophage Interactions.- Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells.- Image-based Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host Response to Microbe Analysis Pipeline.- Metabolomic Analysis of Toxoplasma gondii Tachyzoites.- Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phospho- and Total Proteome in Toxoplasma