Systems Biology of Alzheimer's Disease

Alzheimer's as a Systems-Level Disease Involving the Interplay of Multiple Cellular Networks.- Application of Systems Theory in Longitudinal Studies on the Origin and Progression of Alzheimer's Disease.- The APP Proteolytic System and Its Interactions with Dynamic Networks in Alzheimer's Disease.- Effects of Mild and Severe Oxidative Stress on BACE1 Expression and APP Amyloidogenic Processing.- Advanced Assay Monitoring APP-Carboxyl-Terminal Fragments as Markers of APP Processing in Alzheimer Disease Mouse Models.- Advanced Optical Super-Resolution Method and Techniques for the Study of beta-Amyloid Aggregation In Vitro and In Vivo.- Protocols for Monitoring the Development of Tau Pathology in Alzheimer's Disease.- LC3-II Tagging and Western Blotting for Monitoring Autophagic Activity in Mammalian Cells.- Advanced Mitochondrial Respiration Assay for Evaluation of Mitochondrial Dysfunction in Alzheimer's Disease.- Analysis of Microglial Proliferation in Alzheimer's Disease.- Yeast as aModel for Alzheimer's Disease: Latest Studies and Advanced Strategies.- Yeast as a Model for Studies on Abeta Aggregation Toxicity in Alzheimer's Disease, Autophagic Responses, and Drug Screening.- Drosophila melanogaster as a Model for Studies on the Early Stages of Alzheimer's Disease.- Chronic Mild Stress Assay Leading to Early Onset and Propagation of Alzheimer's Disease Phenotype in Mouse Models.- Gene Expression Studies on Human Trisomy 21 iPSCs and Neurons: Towards Mechanisms Underlying Down's Syndrome and Early Alzheimer's Disease-Like Pathologies.- Cortical Differentiation of Human Pluripotent Cells for In Vitro Modeling of Alzheimer's Disease.- Next Generation Sequencing in Alzheimer's Disease.- Pooled-DNA Sequencing for Elucidating New Genomic Risk Factors, Rare Variants Underlying Alzheimer's Disease.- New Genome-Wide Methods for Elucidation of Candidate Copy Number Variations (CNVs) Contributing to Alzheimer's Disease Heritability.- RNA-Sequencing to Elucidate Early

Stephen G. Oliver

Department of Biochemistry & Cambridge Systems Biology Centre

University of Cambridge

Cambridge UK

steve.oliver@bioc.cam.ac.uk



Juan I. Castrillo

"The book represents a very useful introduction to Systems Biology as the approach necessary to obtain a global view of the dysregulation of pathways involved in AD. In the need for standardization of techniques, the methods described in this book provide a step forward in the definition of common guidelines leading to the reproducible and dependable results necessary for a better understanding of the pathophysiology of AD." (Dr. Alessandro Villa, Journal of Alzheimer's Disease, Vol. 50, 2016)