Synthetic Biology

1 - DNA synthesis and genome engineering 1.1 Competition and the Future of Reading and Writing DNA 1.2 Trackable multiplex recombineering (TRMR) and next-generation genome design technologies 1.3 Site-directed genome alteration with engineered recombinases/ZFs 1.4 Rational efforts to streamline the E. coli genome 1.5 Functional requirements in the program and the cell chassis for next generation synthetic biology 2 - Parts and devices supporting control of protein expression and activity 2.1 Constitutive and regulated promoter engineering 2.2 Splicing and Alternative Splicing Impact on Gene Design 2.3 Application of RNA interference 2.4 RNA switches 2.5 Programming Gene Expression by Engineering Transcript Stability Control and Processing in Bacteria 2.6 Standardized functional peptides and their application in superfunctionalizing proteins 3 - Parts and devices supporting spatial engineering 3.1 Metabolic channeling using DNA as a scaffold 3.2 Synthetic RNA scaffolds for spatial engineering in cells 3.3 Sequestered: Design and Construction of Synthetic Organelles 4 - Early applications of synthetic biology: pathways, therapies, and cell-free synthesis 4.1 Cell free protein synthesis: an emerging technology for understanding, harnessing, and expanding the capabilities of biological systems 4.2 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries 4.3 Synthetic Biology in Immunotherapy 05 - Societal ramifications of synthetic biology Synthetic Biology: From Genetic Engineering 2.0 to Responsible Research and Innovation

Series editors: Sang Yup Lee is Distinguished Professor at the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST). He is currently the Director of the Center for Systems and Synthetic Biotechnology, Director of the BioProcess Engineering Research Center, and Director of the Bioinformatics Research Center. He received numerous awards, including the National Order of Merit, the Merck Metabolic Engineering Award and the Elmer Gaden Award. Lee is the Editor-in-Chief of the Biotechnology Journal and Associate Editor and board member of numerous other journals. Lee is currently serving as a member of Presidential Advisory Committee on Science and Technology (Korea). Jens Nielsen has a PhD degree (1989) in Biochemical Engineering from the Danish Technical University (DTU), and after that established his independent research group and was appointed full Professor there in 1998. He was Fulbright visiting professor at MIT in 1995-1996. At DTU he founded and directed the Center for Microbial Biotechnology. In 2008 he was recruited as Professor and Director to Chalmers University of Technology, Sweden. Jens Nielsen has received numerous Danish and international awards including the Nature Mentor Award, and is member of several academies, including the National Academy of Engineering in USA and the Royal Swedish Academy of Science. He is a founding president of the International Metabolic Engineering Society. Professor Gregory Stephanopoulos is the W. H. Dow Professor of Chemical Engineering at the Massachusetts Institute of Technology (MIT, USA) and Director of the MIT Metabolic Engineering Laboratory. He is also Instructor of Bioengineering at Harvard Medical School (since 1997). He has been recognized by numerous awards from the American Institute of Chemical Engineers (AIChE) (Wilhelm, Walker and Founders awards), American Chemical Society (ACS), Society of industrial Microbiology (SIM), BIO (Washington