SUMO Protocols
(Sprache: Englisch)
Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways. Their influence on eukaryotic cellular metabolism is comparable to that of other...
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Klappentext zu „SUMO Protocols “
Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways. Their influence on eukaryotic cellular metabolism is comparable to that of other modifi- tions such as phosphorylation, acetylation and methylation. The small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself; yet, the functions of the SUMO system are highly diverse and largely independent of the ubiquitin system. SUMO modification controls the activity of tr- scription factors and can influence protein stability, but it also contributes to nucleo-cy- plasmic transport, chromosome segregation and DNA damage repair. As a consequence, the SUMO system pervades virtually all areas of basic molecular and cell biology, and scientists from different backgrounds, including medical researchers, are likely to enco- ter SUMO in the course of their studies. This volume, SUMO Protocols, therefore aims at presenting a collection of methods relevant to SUMO research in order to make these tools available to biochemists, molecular and cell biologists as well as research-oriented clinicians not yet familiar with the system. In contrast to the ubiquitin system, which has been the subject of several reviews and methods collections, no practical compendium entirely devoted to SUMO has been p- lished.
The functions of the ubiquitin-related modifier SUMO are highly diverse and largely independent of the ubiquitin system. In this volume, leading experts present methods that highlight SUMO's unique biological features in order to support future SUMO research.
Inhaltsverzeichnis zu „SUMO Protocols “
Part I. Introduction1. The SUMO System: An Overview Helle D. Ulrich
Part II. Identification of SUMO Targets
2. Identification of SUMO Target Proteins by Quantitative Proteomics Jens S. Andersen, Ivan Matic and Alfred C. O. Vertegaal
3. Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry James A. Wohlschlegel
4. Identification of SUMO Targets through In Vitro Expression Cloning Christian B. Gocke and Hongtao Yu
5. Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS) Rainer Niedenthal
6. In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae Helle D. Ulrich and Adelina A. Davies
Part III. Identification of SUMO Interactors
7. Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis Mary B. Kroetz and Mark Hochstrasser
8. Identification of SUMO Binding Motifs by NMR Candace S. Seu and Yuan Chen
Part IV. Functional Analysis of Sumoylation In Vivo
9. Regulation of Transcription Factor Activity by SUMO Modification Jian Ouyang, Alvaro Valin and Grace Gill
10. Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization Shanshan Zhu and Michael J. Matunis
Part V. Biochemical Studies of SUMO Conjugation
11. Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation Ali A. Yunus and Christopher D. Lima
12. Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes Andreas Werner, Marie-Christine Moutty, Ulrike Möller and Frauke Melchior
13. Preparation of Sumoylated Substrates for Biochemical Analysis Puck Knipscheer, Helene Klug, Titia K. Sixma and Andrea Pichler
14. Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli Hisato Saitoh, Junsuke Uwada and Azusa Kawasaki
Part VI. Biochemical Studies of SUMO Deconjugation
15. Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous
... mehr
Substrates David Reverter and Christopher D. Lima
16. An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage Nicolas Stankovic-Valentin, Lukasz Kozaczkiewicz, Katja Curth and Frauke Melchior
17. FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities Michael H. Tatham and Ronald T. Hay
18. Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay Craig A. Leach, Xufan Tian, Michael R. Mattern and Benjamin Nicholson
Part VII. Manipulation and Alternative Uses of the SUMO System
19. Inhibition of the SUMO Pathway by Gam1 Mariaelena Pozzebon, Chiara V. Segre and Susanna Chiocca
20. SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems Tadas Panavas, Carsten Sanders and Tauseef R. ButtR />Part VI. Biochemical Studies o
16. An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage Nicolas Stankovic-Valentin, Lukasz Kozaczkiewicz, Katja Curth and Frauke Melchior
17. FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities Michael H. Tatham and Ronald T. Hay
18. Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay Craig A. Leach, Xufan Tian, Michael R. Mattern and Benjamin Nicholson
Part VII. Manipulation and Alternative Uses of the SUMO System
19. Inhibition of the SUMO Pathway by Gam1 Mariaelena Pozzebon, Chiara V. Segre and Susanna Chiocca
20. SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems Tadas Panavas, Carsten Sanders and Tauseef R. ButtR />Part VI. Biochemical Studies o
... weniger
Bibliographische Angaben
- 2009, 336 Seiten, Masse: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Helle Ulrich
- Verlag: Humana Press
- ISBN-10: 1934115800
- ISBN-13: 9781934115800
- Erscheinungsdatum: 23.10.2008
Sprache:
Englisch
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