SH2 Domains

Introduction: History of SH2 Domains and Their Applications.- What Have We Learned from SH2 Domains?.- Hidden Markov Models for Protein Domain Homology Identification and Analysis.- Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes.- SH2 Ligand Prediction-Guidance for In-Silico Screening.- An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions.- Proteomic Clustering Analysis of SH2 Domain Datasets.- Expression and Production of SH2 Domain Proteins.- Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay.- Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies.- Expression and Purification of SH2 Domains Using Baculovirus Expression System.- Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation.- Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain.- Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain.- NMR Chemical Shift Mapping of SH2 Peptide Interactions.- Calorimetric Measurement of SH2 Domain Ligand Affinities.- Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization.- In-Solution SH2 Domain Binding Assay Based on Proximity Ligation.- Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists.- Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays.- High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays.- SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis.- Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry.- Analysis of the Global Changes in SH2 Binding

Kazuya Machida, MD, PhD Dept of Genetics and Developmental Biology Univ of Connecticut Health Center Cell and Genome Sciences, Farmington, CT, USA
Tel: 860-679-1830; Email: machida@neuron.uchc.edu