Regulatory B Cells
Methods and Protocols
(Sprache: Englisch)
Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties....
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Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
Inhaltsverzeichnis zu „Regulatory B Cells “
Part I: Purification and Phenotypic Characterization of B Cell Subsets1. Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells
M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti
2. Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice
Vanessa Yenson and Nicole Baumgarth
3. Purification and Immunophenotypic Characterization of Murine B10 B Cells
Chao Hong and Xiao-Ming Gao
4. Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions
Jean-David Bouaziz, Hélène Le Buanec, and Adèle de Masson
Part II: Mechanisms of Immune Suppression by B Cells
5. IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques
Jun Deng, Xiang Lin, and Liwei Lu
6. TGF-? Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques
Yoshiyuki Mishima, Shunji Ishihara, Jonathan J. Hansen, and Yoshikazu Kinoshita
7. Characterization and Activity of Fas Ligand Producing CD5+ B Cells
Steven K. Lundy and Matthew W. Klinker
Part III: Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells
8. Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties
Elisabeth Calderón-Gómez and Simon Fillatreau
9. Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines
Aurélie Tormo, Jiusheng Deng, Edouard Al-Chami, Ziouani Sonia, and Moutih Rafei
10. Toll-Like Receptor Ligation for the Induction of Regulatory B Cells
Luciën E.P.M van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E.D Martha, and Hermelijn H. Smits
11. The Generation of Regulatory B Cells by Helminth Parasites
Adnan Khan, Sylvie Amu, Sean P. Saunders, and Padraic G. Fallon
12. Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions
Francesca Mion, Federica Benvenuti, Carlo Pucillo, and Gaetano Vitale
Part
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IV: Study of Regulatory B Cells in Pathological Settings
13. Regulatory B Cells in Experimental Mouse Models of Arthritis
Elizabeth C. Rosser and Claudia Mauri
14. Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE)
Takashi Matsushita
15. Regulatory B Cells in Allergic Airways Disease and Asthma
Prabitha Natarajan, Linda A. Guernsey, and Craig M. Schramm
16. Regulatory B Cells in Mouse Models of Intestinal Inflammation
Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, and Atsushi Mizoguchi
17. Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE)
Avijit Ray and Sreemanti Basu
18. Regulatory B Cells, Helminthes, and Multiple Sclerosis
Jorge Correale and Tomas Rivero Equiza
19. Generation and Identification of Tumor-Evoked Regulatory B Cells
Arya Biragyn, Catalina Lee-Chang, and Monica Bodogai
13. Regulatory B Cells in Experimental Mouse Models of Arthritis
Elizabeth C. Rosser and Claudia Mauri
14. Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE)
Takashi Matsushita
15. Regulatory B Cells in Allergic Airways Disease and Asthma
Prabitha Natarajan, Linda A. Guernsey, and Craig M. Schramm
16. Regulatory B Cells in Mouse Models of Intestinal Inflammation
Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, and Atsushi Mizoguchi
17. Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE)
Avijit Ray and Sreemanti Basu
18. Regulatory B Cells, Helminthes, and Multiple Sclerosis
Jorge Correale and Tomas Rivero Equiza
19. Generation and Identification of Tumor-Evoked Regulatory B Cells
Arya Biragyn, Catalina Lee-Chang, and Monica Bodogai
... weniger
Bibliographische Angaben
- 2014, 2014, XI, 292 Seiten, 19 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Gaetano Vitale, Francesca Mion
- Verlag: Springer, Berlin
- ISBN-10: 1493911600
- ISBN-13: 9781493911608
- Erscheinungsdatum: 14.07.2014
Sprache:
Englisch
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