Recombinant Proteins from Plants

Part I: Vectors and Transformation

1. Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology

Johanna Gottschamel and Andreas Lössl  

2. Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors

Ruifeng He

3. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, and George P. Lomonossoff

4. Transient Protein Expression by Agroinfiltration In Lettuce

Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa Mcnulty, Kahlin Leuzinger, Huafang Lai

5. Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii

Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena Almaraz-Delgado, Noé Durán-Figueroa, and Jesus Agustín Badillo-Corona

Part II: Product Improvements

6. Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini

7. Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation  

Alexandra Castilho and Herta Steinkellner

8. Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

Stéphanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-André D'Aoust, Marie-Claire Goulet, Frank Sainsbury and Dominique Michaud 

Part III: Extraction and Scale-Up

9. Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector

Lauren Moore, Krystal Hamorsky, and Nobuyuki Matoba

10. Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots 

Manish L. Raorane, Joan O. Narciso, and Ajay Kohli

11. Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In