Rapid Diagnosis of Dengue Outbreaks in Resource Limited Facilities

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CHAPTER 5: DENGUE VIRAL INFECTION AND HOST FACTORS:
Dengue Viral infection:
Dengue virus binds to its receptor mediated by E protein after which it is fused into acidic lysosomes through receptor-mediated endocytosis [37]. Dengue virus enters Langerhans cells of skin through binding between viral proteins and membrane proteins, specifically the C-type lectins such as DC-SIGN/L-SIGN, mannose receptor, heparan sulfate, nLc4Cer and CLEC5A. DEN-2 also binds with HSP70/HSP90, GRP78, CD14-associated protein and two unknown proteins having trypsin resistance and trypsin sensitive properties. DEN1-3 serotypes bind with Laminin receptor while DEN2-4 serotypes bind with an unknown protein as well [24, 38, 39]. The virus genome is translated in membrane bound vesicles on endoplasmic reticulum and new viral proteins replicate viral RNA. Immature virus particles are transported to Golgi apparatus and get released by exocytosis through budding on maturation and infect monocytes and macrophages subsequently. In only few hours after infection, thousands of copies of viral molecules are produced from a single viral molecule leading to cell damage. Viral-encoded RNA-dependent RNA polymerases and other cellular factors are responsible for catalyzing the infection cycle of dengue virus [24]. The viral titres peak around the onset of clinical features, a time when the infection is widespread.
Host Factors:
DF is an important cause of pediatric hospitalization in SEAR. Since 1980s, studies have reported a higher association of DHF with older ages [24, 32, 45, 51]. From 1990-96, the highest age-specific morbidity rates were in the 15 to 34 year age group. Males are commonly infected, although females are prone to severe disease possibly due to accelerated immune response [40-42]. Severe dengue has been observed less frequently in dengue infected black persons than whites [25]. Genetic polymorphisms increasing dengue risk include polymorphisms in genes coding for TNF ,