Pyrosequencing

The History of PyrosequencingÒ.- Pyro Mark® Instruments, Chemistry and Software for Pyrosequencing® Analysis.- Software-Based Pyrogram® Evaluation.- Quantitative Validation and Quality Control of Pyrosequencing® Assays.- Extended KRAS and NRAS Mutation Profiling by Pyrosequencing®.- Universal BRAF State Detection by the Pyrosequencing®-based U BRAFV600 Assay.- Pyrosequencing®-based Identification of Low Frequency Mutations Enriched through Enhanced-ice-COLD-PCR.- Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing®.- Analysis of Copy Number Variation By Pyrosequencing® using Paralogous Sequences.- Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing®.- HLA-B and HLA-C Supratyping by Pyrosequencing®.- Allele Quantification Pyrosequencing® at Designated SNP sites to Detect Allelic Expression Imbalance and Loss-of-heterozygosity.- Quantitative DNA Methylation Analysis by Pyrosequencing®.- Quantitative Methylation Analysis of the PCDHB Gene Cluster.- Assessment of Changes in global DNA Methylation Levels by Global Analysis of DNA 5-methylcytosine using the Luminometric Methylation Assay, LUMA.- Limiting Dilution Bisulfite Pyrosequencing®: a Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells.- Detection of Loss of Imprinting by Pyrosequencing®.- Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing®.- Allele-specific DNA methylation detection by Pyrosequencing®.- SNP-based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing®.- DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution byPyrosequencing®.- Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions.-Detection of Drug-Resistant Mycobacterium tuberculosis.- Application of Pyrosequencing® in Food Biodefense.- Forensic Analysis of Mitochondrial and Autosomal Markers using Pyrosequencing®.- Tissue-specific DNA

Prof. Dr. Ulrich Lehmann lehrte bis zu seiner Emeritierung am Geologisch-Paläontologischen Institut der Universität Hamburg.