Protein Terminal Profiling

[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay.- DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.- SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation.- Profiling of Protein N-Termini and their Modifications in Complex Samples.- Protease Substrate Profiling by N-Terminal COFRADIC.- Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification.- Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types.- System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates.- Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC.- ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling.- Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP).- Multiplexed Protease Specificity Profiling Using Isobaric Labeling.- FPPS - Fast Profiling of Protease Specificity.- Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics.- Prediction of Proteases Involved in Peptide Generation.- Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches.- Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors.- Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method.- Synthesis and Application of Activity-Based Probes for Proteases.

Dipl.-Psych. Oliver Schilling lehrt an der Universität Heidelberg.