Protein Kinase Protocols

Protein Kinase-Mediated Signaling Networks: Regulation and Functional Characterization, Alastair D. Reith. Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies, Lisa D. Chong and Ira O. Daar. Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins, Kathleen M. Woods-Ignatoski. Two-Dimensional Phosphoamino Acid Analysis, Peter Blume-Jensen and Tony Hunter. Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases, Jeremy M. Tavaré and Tarik Issad. Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry, A. Grey Craig. Phosphorylation of Smad Signaling Proteins by Receptor Serine/ Threonine Kinases, Serhiy Souchelnytskyi, Lars Rönnstrand, Carl-Henrik Heldin, and Peter ten Dijke. Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate, Rozen Le Panse, Navita Rampersaud, and Louis C. Mahadevan. JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins: Analysis by Phosphotyrosine Blotting and EMSA, Nicola Broughton and Mark S. Burfoot. Assays for Glycogen Synthase Kinase-3 (GSK-3), Darren Cross. Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements, Gavin Brooks. Protein Histidine Kinase, Harry R. Matthews and Karina Chan. Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System, Tracey Purton, Sandra Wilkinson, and Edward J. Murray. Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins, Ralph A. Zirngibl and Peter Greer. Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay, Mary Rose Burnham, Regina DeBerry, and Amy H. Bouton. Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries, Pier

"By engaging high-ranking experts as authors of the individual chapters the editor has succeeded in compiling an updated an highly useful compendium of today's technology in protein kinase research. besides its topicality, this book has the particular advantage that the chapters are arranged according to a constantly recurring scheme, that is, beginning with a comprehensive introduction, which provides the appropriate key references, and then continuing with very detailed experimental instructions, which are given clearly enough to enable even a less experienced experimentator to follow. The "Notes" at the end of each chapter are extremely helpful, in which the experimental steps are critically examined and explained in detail....for students and newcomers in the field it provides an excellent introduction to the methodology of protein kinase research, and for the experienced scientist it will be a highly valuable work of reference and an indispensable companion in daily laboratory work." - Chembiochem

"The authors are all experienced researchers with some eminent names among them. Each of the chapters has been contributed by scientists with considerable expertise in the areas included, leaving the reader with knowledge that the protocols are thoroughly tried and tested. All in all, the book presents a wide range of specialized techniques which those working on (or intending to work on) kinases would find a very useful addition to their laboratories." - Cell Biology International