Plant Signalling Networks

Experimental Analysis of Receptor Kinase Phosphorylation.- Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies.- Identification of O-GlcNAc Modified Proteins from Arabidopsis.- Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE).- Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE).- Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana.- Small-Molecule Dissection of Brassinosteroid (BR) Signaling.- A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination.- Activation Tagging.- Rho GTPase Activity Analysis in Plant Cells.- Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy.- In vivo Ubiquitination Assay by Agroinfiltration.- In Vitro Protein Ubiquitination Assay.- Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq).- Smart Pooling of mRNA Samples for Efficient Transcript Profiling.- Transient Expression Assays for Quantifying Signaling Output.- Genome-Wide Profiling of Uncapped mRNA.- Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches.

Zhenbiao Yang is Professor in Plant Cell Biology, University of California, Riverside. He is a world authority on plant intracellular signaling mediated by the Rho family of small G proteins and a leader in signaling in polarized cell growth and cell morphogenesis in plants.

From the reviews:

"This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user." (Peter Schröder, Plant Science Bulletin, Vol. 59 (1), 2013)

From the reviews:"This protocol book is a good overview across various research approaches such as genomics, proteomics, metabolomics, and bioinformatics. Its methods are elaborate and clear, and most of the chapters provide specific hints and notes to increase the success of the experiments. This book will long stand as a guide to plant signaling networks, and it can be taken as a useful vademecum to the lab for the more experienced user." (Peter Schröder, Plant Science Bulletin, Vol. 59 (1), 2013)