Plant Developmental Biology
Methods and Protocols
(Sprache: Englisch)
The beauty and variety of plants has fascinated mankind for thousands of years. In this comprehensive and revolutionary book, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology.
Jetzt vorbestellen
versandkostenfrei
Buch (Gebunden)
Fr. 177.00
inkl. MwSt.
- Kreditkarte, Paypal, Rechnungskauf
- 30 Tage Widerrufsrecht
Produktdetails
Produktinformationen zu „Plant Developmental Biology “
The beauty and variety of plants has fascinated mankind for thousands of years. In this comprehensive and revolutionary book, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology.
Klappentext zu „Plant Developmental Biology “
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant's life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.
The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant's life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.
Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.
Inhaltsverzeichnis zu „Plant Developmental Biology “
- Growth protocols for model plants in developmental biology- Grafting as a research tool
- Viral-induced gene silencing as a reverse genetics tool to study gene function
- The CRE/lox system as a tool for developmental studies at the cell and tissue level
- Inducible gene expression systems for plants
- Trichome development in Arabidopsis
- Phenotyping the development of leaf area in Arabidopsis thaliana
- Analyzing shoot apical meristem development
- Analyzing floral meristem development
- Female gametophytic mutants: Diagnosis and characterization
- Pollen tube development
- Analysis of root meristem size development
- Phenotypic characterization of photomorphogenic responses during plant development
- Kinematic analysis of cell division and expansion
- Flowering time control
- mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in Arabidopsis
- Immunolocalization of proteins in plants
- Detection of small non-coding RNAs
- Quantitative real time PCR in plant developmental biology
- Luciferase and green fluorescent protein reporter genes as tools to determine protein abundance and intracellular dynamics
- Fluorescence-activated cell sorting in plant developmental biology
- Laser microdissection of paraffin embedded plant tissues for transcript profiling
- Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plants
- The split luciferase complementation assay
- Co-immunoprecipitation and protein blots
- Probing protein-protein interactions with FRET-FLIM
- Plant chromatin immunoprecipitation
- Immunocytological analysis of chromatin in isolated nuclei
- Bisulphite sequencing of plant genomic DNA
Bibliographische Angaben
- 2010, XI, 448 Seiten, Masse: 18,6 x 26,3 cm, Gebunden, Englisch
- Herausgegeben: Lars Hennig, Claudia Köhler
- Verlag: Springer, Berlin
- ISBN-10: 1607617641
- ISBN-13: 9781607617648
Sprache:
Englisch
Kommentar zu "Plant Developmental Biology"
0 Gebrauchte Artikel zu „Plant Developmental Biology“
| Zustand | Preis | Porto | Zahlung | Verkäufer | Rating |
|---|
Schreiben Sie einen Kommentar zu "Plant Developmental Biology".
Kommentar verfassen