Nucleic Acid and Peptide Aptamers
Methods and Protocols
(Sprache: Englisch)
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and...
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After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology(TM) series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
Klappentext zu „Nucleic Acid and Peptide Aptamers “
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology(TM) series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
Inhaltsverzeichnis zu „Nucleic Acid and Peptide Aptamers “
Part I.) Nucleic Acid Aptamers1. Nucleic Acid Pool Preparation and Characterization
Shawn K. Piasecki, Bradley Hall, and Andrew D. Ellington
2. In vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins
Günter Mayer and Thomas Höver
3. Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX)
Renee Mosing and Michael T. Bowser
4. In vitro Selection of Allosteric Ribozymes
Nicolas Piganeau
5. Cell-Specific Aptamers for Targeted Therapies
Laura Cerchia, Paloma H. Giangrande, James O. McNamara, and Vittorio de Franciscis
6. Aptamers Targeting RNA Molecules
Marguerite Watrin, Eric Dausse, Isabelle Lebars, Bernard Rayner, Anthony Bugaut, and Jean-Jaques Toulmé
7. Fluorescence Correlation Spectroscopy (FCS) Based Characterization of Aptamer Ligand Interaction
Arne Werner and Uli Hahn
8. Structural Probing Techniques on Natural Aptamers
Catherine A. Wakeman and Wade C. Winkler
9. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography
Andrea L. Edwards, Andrew D. Garst, and Robert T. Batey
10. Locked Nucleic Acid Aptamers
Jan Barciszewski, Michael Medgaard, Troels Koch, Jens Kurreck, and Volker A. Erdmann
11. Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay
Satoko Yamazaki and Michael Famulok
12. Aptamers as Artificial Gene Regulation Elements
Beatrix Suess and Julia E. Weigand
13. Aptamers and Biosensors
Thomas M. A. Gronewold
14. Nanoparticles/Dip Stick
Yi Lu, Juewen Liu, and Debapriya Mazumdar
15. In vivo Imaging of Oligonucleotidic Aptamers
Bertrand Tavitian, Frédéric Ducongé, Raphaël Boisgard and Frédéric Dollé Tavitian
Part II.) Peptide Aptamers
16. Selection of Peptides Interfering with Protein-Protein Interaction
Annette Gaida, Urs B. Hagemann, Dinah Mattay, Christina Räuber, Kristian M. Müller, and Katja M. Arndt
17. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display
Terry T. Takahashi
... mehr
and Richard W. Roberts
18. Ligand-Regulated Peptide Aptamers
Russell A. Miller
19. Isolation of Peptide Aptamers to Target Protein Function
Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, and Linda Hanley-Bowdoin
20. Microbodies(TM)
Hans-Ulrich Schmoldt, Matin Daneschdar, Harald Kolmar, and Michael Blind
21. Peptide Aptamers for Small Molecule Drug Discovery
Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, and Pierre Colas
22. Synthesis and Application of Peptides as Drug Carriers
Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger
18. Ligand-Regulated Peptide Aptamers
Russell A. Miller
19. Isolation of Peptide Aptamers to Target Protein Function
Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, and Linda Hanley-Bowdoin
20. Microbodies(TM)
Hans-Ulrich Schmoldt, Matin Daneschdar, Harald Kolmar, and Michael Blind
21. Peptide Aptamers for Small Molecule Drug Discovery
Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, and Pierre Colas
22. Synthesis and Application of Peptides as Drug Carriers
Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger
... weniger
Autoren-Porträt
Günter Mayer, Justizoberamtsrat i. R., war Rechtspfleger beim Vollstreckungsgericht Kaiserslautern und Dozent an der FH Schwetzingen, einer Hochschule für Rechtspflege. Er verfügt über das Fachwissen und die nötige Erfahrung in allen Fragen der Eigentumsübertragung von Grundbesitz.
Bibliographische Angaben
- 2009, XI, 408 Seiten, Masse: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Günter Mayer
- Verlag: Springer, Berlin
- ISBN-10: 1934115894
- ISBN-13: 9781934115893
- Erscheinungsdatum: 26.03.2009
Sprache:
Englisch
Rezension zu „Nucleic Acid and Peptide Aptamers “
From the reviews:
Pressezitat
From the reviews: "The book 'Nucleic Acids and Peptide Aptamers' is a collection of detailed protocols describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. ... The list of references is a useful and timely source of further reading on the subject. ... can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)
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