Nuclear G-Protein Coupled Receptors
Methods and Protocols
(Sprache: Englisch)
Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine...
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Klappentext zu „Nuclear G-Protein Coupled Receptors “
Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.
Inhaltsverzeichnis zu „Nuclear G-Protein Coupled Receptors “
1. Studies of Intracellular Angiotensin IIRichard N. Re and Julia L. Cook
2. Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes
Clémence Merlen and Jonathan Ledoux
3. Design and Application of Light-Activated Probes for Cellular Signaling
David Chatenet, Steve Bourgault, and Alain Fournier
4. Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes
Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen
5. Quantification of Catecholamine Uptake in Adult Cardiac Myocytes
Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell
6. Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase
Chantal Binda and Jean-Luc Parent
7. Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes
Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel
8. High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs)
Vikrant K. Bhosle, Fernand Gobeil Jr., Jose Carlos Rivera, Alfredo Ribeiro-da-Silva, and Sylvain Chemtob
9. Biochemical Fractionation of Membrane Receptors in the Nucleus
Ying-Nai Wang, Longfei Huo, Jennifer L. Hsu, and Mien-Chie Hung
10. Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons
Yuh-Jiin I. Jong and Karen L. O'Malley
11. Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors
Karen A. Ryall and Jeffrey J. Saucerman
12. Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]
Senka Ljuboyevi? and Donald M. Bers
13. Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors
Julie Bossuyt and Donald M. Bers
14. Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting
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Proteins
Rhiannon Campden, Darlaine Pétrin, Mélanie Robitaille, Nicolas Audet, Sarah Gora, Stéphane Angers, and Terence E. Hébert
15. Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei
George Vaniotis, Sarah Gora, André Nantel, Terence E. Hébert, and Bruce G. Allen
16. Trafficking and Function of GPCRs in the Endosomal Compartment
Davide Calebiro, Amod Godbole, Sandra Lyga, and Martin J. Lohse
Rhiannon Campden, Darlaine Pétrin, Mélanie Robitaille, Nicolas Audet, Sarah Gora, Stéphane Angers, and Terence E. Hébert
15. Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei
George Vaniotis, Sarah Gora, André Nantel, Terence E. Hébert, and Bruce G. Allen
16. Trafficking and Function of GPCRs in the Endosomal Compartment
Davide Calebiro, Amod Godbole, Sandra Lyga, and Martin J. Lohse
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Bibliographische Angaben
- 2014, 2015, XV, 215 Seiten, 15 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Bruce G. Allen, Terence E. Hébert
- Verlag: Springer, Berlin
- ISBN-10: 1493917544
- ISBN-13: 9781493917549
- Erscheinungsdatum: 11.10.2014
Sprache:
Englisch
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