Neutrophil Methods and Protocols
(Sprache: Englisch)
This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written...
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Klappentext zu „Neutrophil Methods and Protocols “
This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.
Inhaltsverzeichnis zu „Neutrophil Methods and Protocols “
- Neutrophils- Methods and Protocols
- Edited by
... Mark T. Quinn
... Frank R. DeLeo
... Gary M. Bokoch
- Contents
- Dedication i
- Preface ii
- Contributors v
PART I: NEUTROPHILS: AN OVERVIEW
1. The Role of Neutrophils in the Immune System: An Overview
- Harry L. Malech
PART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION
2. Isolation of Human Neutrophils From Venous Blood
- William M. Nauseef
3. Neutrophil Isolation From Non-Human Species
- Daniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn
4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
- Lene Udby and Niels Borregaard
PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS
5. Rac and Rap GTPase Activation Assays
- Ulla G. Knaus, Alison Bamberg, and Gary M. Bokoch
6. Measurement of Phospholipid Metabolism in Intact Neutrophils
- Susan Sergeant and Linda C. McPhail
7. Analysis of Protein Phosphorylation in Human Neutrophils
- Jamel El-Benna and Pham My-Chan Dang
8. Mitogen-Activated Protein Kinase Assays
- Martine Torres
9. Transduction of Proteins into Intact Neutrophils
- Tieming Zhao and Gary M. Bokoch
10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
- Esther J. Hillson, Sharon Dewitt, and Maurice B. Hallett
11. Analysis of Electrophysiological Properties and Responses of Neutrophils
- Deri Morgan and Thomas E. DeCoursey
12. Analysis of Neutrophil Apoptosis
- Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. Hart
PART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS
13. Optical and Fluorescence Detection of Neutrophil Integrin Activation
- Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon
14. Analysis of Neutrophil Polarization and Chemotaxis
- Mary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher
15. Quantifying and Localizing Actin Free Barbed Ends in
... mehr
Neutrophils
- Michael Glogauer
16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
- Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda
17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
- Nancy A. Louis, Eric Campbell, and Sean P. Colgan
PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY
18. Immunofluorescence and Confocal Microscopy of Neutrophils
- Lee-Ann H. Allen
19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
- Benjamin E. Steinberg and Sergio Grinstein
20. Analysis of Neutrophil Membrane Traffic During Phagocytosis
- Per Lönnbro, Pontus Nordenfelt, and Hans Tapper
21. Analysis of Neutrophil Bactericidal Activity
- Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton
22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
- Per Follin and Claes Dahlgren
PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES
23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes
- Claes Dahlgren, Anna Karlsson, and Johan Bylund
24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
- Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez
25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly
- Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick
26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics
- Ross M. Taylor and Algirdas J. Jesaitis
PART VII. ANALYSIS OF NEUTROPHIL GENE EXPRESSION AND TRANSCRIPTION FACTORS
27. Genome-scale Transcript Analyses with Human Neutrophils
- Scott D. Kobayashi, Dan E. Sturdevant, and Frank R. DeLeo
28. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
- Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni
29. Detection of Intact Transcription Factors in Human Neutrophils
- Patrick P. McDonald and Richard D. Ye
PART VIII. NEUTROPHIL DEFECTS AND DIAGNOSIS
30. Disorders of Neutrophil Function: An Overview
- Mary C. Dinauer
31. Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
- Houda Zghal Elloumi and Steven M. Holland
32. Diagnostic Assays for Myeloperoxidase Deficiency
- William M. Nauseef
- Michael Glogauer
16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
- Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda
17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
- Nancy A. Louis, Eric Campbell, and Sean P. Colgan
PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY
18. Immunofluorescence and Confocal Microscopy of Neutrophils
- Lee-Ann H. Allen
19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
- Benjamin E. Steinberg and Sergio Grinstein
20. Analysis of Neutrophil Membrane Traffic During Phagocytosis
- Per Lönnbro, Pontus Nordenfelt, and Hans Tapper
21. Analysis of Neutrophil Bactericidal Activity
- Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton
22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
- Per Follin and Claes Dahlgren
PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES
23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes
- Claes Dahlgren, Anna Karlsson, and Johan Bylund
24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
- Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez
25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly
- Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick
26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics
- Ross M. Taylor and Algirdas J. Jesaitis
PART VII. ANALYSIS OF NEUTROPHIL GENE EXPRESSION AND TRANSCRIPTION FACTORS
27. Genome-scale Transcript Analyses with Human Neutrophils
- Scott D. Kobayashi, Dan E. Sturdevant, and Frank R. DeLeo
28. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
- Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni
29. Detection of Intact Transcription Factors in Human Neutrophils
- Patrick P. McDonald and Richard D. Ye
PART VIII. NEUTROPHIL DEFECTS AND DIAGNOSIS
30. Disorders of Neutrophil Function: An Overview
- Mary C. Dinauer
31. Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
- Houda Zghal Elloumi and Steven M. Holland
32. Diagnostic Assays for Myeloperoxidase Deficiency
- William M. Nauseef
... weniger
Bibliographische Angaben
- 2007, 537 Seiten, mit farbigen Abbildungen, Masse: 16 x 24,1 cm, Gebunden, Englisch
- Herausgegeben:Quinn, Mark T.; DeLeo, Frank R.; Bokoch, Gary M.
- Herausgegeben: Mark T. Quinn, Gary M. Bokoch, Frank R. DeLeo
- Verlag: Humana Press
- ISBN-10: 1588297888
- ISBN-13: 9781588297884
- Erscheinungsdatum: 02.08.2007
Sprache:
Englisch
Rezension zu „Neutrophil Methods and Protocols “
From the reviews:"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars"This excellent book is one of the latest in the 'Methods in Molecular Biology' series ... . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils - whether in research or diagnostics - to purchase one immediately. ... I was impressed by this book's comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)
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