Natural Killer Cells
Methods and Protocols
(Sprache: Englisch)
This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells...
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This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors,electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.
Inhaltsverzeichnis zu „Natural Killer Cells “
TrackingEffector and Memory NK Cells During MCMV Infection.- Applicationof Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural KillerCells.- Assessmentof NK Cell Metabolism.- Genotyping Single Nucleotide Polymorphisms andCopy Number Variability of the FCGRs Expressed on NK Cells.- Measurementof Average Telomere Length in Ex VivoExpanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH).- InVitro Assessment of Human Natural Killer Cell Migration and Invasion.- Microfluidic-BasedLive-Cell Analysis of NK-Cell Migration In Vitro.- Microwell-BasedLive Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and CytotoxicResponse.- Assessmentof Natural Killer Cell Cytotoxicity Using Image Cytometry Method.- Analysisof Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry.- Using NK Cell Lipid Raft Fractionation to Understandthe Role of Lipid Rafts in NK Cell Receptor Signaling.- High-and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse.- ThePlanar Lipid Bilayer System Serves as a Reductionist Approach for Studying NKCell Immunological Synapses and their Functions.- Expansionof NK Cells Using Genetically Engineered K562 Feeder Cells.- Ex-VivoExpansion of Human NK Cells Using K562 Engineered to Express Membrane BoundIL21.- Large-ScaleCulture and Genetic Modification of Human Natural Killer Cells for CellularTherapy.- GeneModification of Human Natural Killer Cells Using a Retroviral Vector.- Modificationof Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive CellularTherapy.- mRNATransfection to Improve NK Cell Homing to Tumors.- InVitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor throughDifferentiation of Gene-Modified Hematopoietic Stem Cells.- EngineeringReceptor Expression on Natural Killer Cells through Trogocytosis.- Electroporationof siRNA to
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Silence Gene Expression in Primary NK Cells.- Mouse Xenograft Model for Intraperitoneal Administrationof NK Cell Immunotherapy for Ovarian Cancer.- AerosolDelivery of Interleukin-2 in Combination with Adoptive Transfer of NaturalKiller Cells for the Treatment of Lung Metastasis: Methodology and Effect.- Non-Invasive Imaging of Natural Killer Cell-MediatedApoptosis in a Mouse Tumor Model.- Non-InvasiveIn Vivo Fluorescence Imaging of NK Cellsin Pre-Clinical Models of Adoptive Immunotherapy.- In Vivo 19F-MagneticResonance Imaging of Adoptively Transferred NK Cells.- Generationof BiKEs and TriKEs to Improve NK Cell-Mediated Targeting of Tumor Cells.- RegulatoryConsiderations for NK Cells Used in Human Immunotherapy Applications.
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Bibliographische Angaben
- 2016, 1st ed. 2016, XV, 365 Seiten, 49 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Srinivas S. Somanchi
- Verlag: Springer, Berlin
- ISBN-10: 1493936824
- ISBN-13: 9781493936823
- Erscheinungsdatum: 13.05.2016
Sprache:
Englisch
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