Mycobacteria Protocols

Culturing Mycobacteria.- DNA Isolation from Mycobacteria.- Extraction and Separation of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria.- Macrophage Infection Models for Mycobacterium tuberculosis.- Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics.- Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy.- Measuring Efflux and Permeability in Mycobacteria.- Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria.- Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells.- Electroporation of Mycobacteria.- Gene Switching and Essentiality Testing.- Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.- Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action.- CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria.- Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology.- Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes.- Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences.- Whole Genome Sequencing of Mycobacterium tuberculosis Directly from Sputum Samples.- Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis: From Total RNA to Differentially Expressed Genes.- RNA Sequencing for Transcript 5'-End Mapping in Mycobacteria.- Methods for Proteomic Analyses of Mycobacteria.- Targeted Lipidomics of Mycobacterial Lipids and Glycolipids.- Metabolomics of Mycobacterium tuberculosis.- Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium.- A Multi-Stress Model for High Throughput Screening Against Non-Replicating