Mucins

Preparation of Jellyfish Mucins.- Extraction of Mucin from Rodent Feces and Determination of O-Linked Oligosaccharide Chain Equivalent Derived from Fecal Mucin.- Preparation of Soluble Mucin Solutions from the Salivary Glands.- Isolation of Membrane Bound Mucins from Human Bronchial Epithelial Cells.- Extraction and Fractionation of Human Gastric Mucins from Gastric Juice.- Extraction of Mucins from the Mammalian Intestinal Tract.- Supported Molecular Matrix Electrophoresis.- Supported Immunohistochemical Staining of Mucins.- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Di uoride Membrane.- Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane.- Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry.- Preparation of O-Glycans from Mucins using Hydrazine Treatment.- Eliminative Oximation of O-glycans from Mucins.- 9-Fluorenylmethyl Chloroformate Labeling forO-Glycan Analysis.- Liquid Chromatography and Capillary Electrophoresis Analysis of 2AA-Labeled O-Glycans.- Preparation of Mucin Glycopeptides by Organic Synthesis.- MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins.- Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass Spectrometry.- Differential Glycoform Analysis of MUC1 Derived from Biological Specimens using an Antibody-Overlay Lectin Microarray.- ISOGlyP: O-Glycosylation Site Prediction using Peptide Sequences and GALNTs.- Assessment of Mucin-Associated Gene Expression Levels on the Ocular Surface.- Methylation Specific Electrophoresis.- Expression Analysis of Genes Corresponding to Mucins and their Glycans from Cervical Tissue using RNA Sequencing.- Recombinant Production of Glycoengineered Mucins in HEK293-F Cells.- Analysis of the Interaction between Mucin and Green Fluorescent Protein (GFP)-Tagged Galectin-2 using a