Lipid Signaling Protocols

Lipid Systems and Techniques.- Eicosanoids: Generation and Detection in Mammalian Cells.- Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry.- Measurement of Polyphosphoinositides in Cultured Mammalian Cells.- Inositol Lipid-Dependent Functions in : Analysis of Phosphatidylinositol Phosphates.- Methods for the Determination of the Mass of Nuclear PtdIns4, PtdIns5, and PtdIns(4,5).- Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin.- Solution and Solid-State NMR of Lipids.- Spatial Localization of PtdIns in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain.- Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes.- Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes.- Effects of Sterols on the Development and Aging of.- Proteomic Analysis of the Sterol-Mediated Signaling Pathway in.- Lipid Metabolism and Recognition.- Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt.- Measurement of PTEN Activity by Imaging Phosphorylated Akt.- Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by and Assays.- Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic #x03B2;-Cells and Insulin-Responsive Muscle Cells and Adipocytes.- Protein Kinase C as an Effector of Lipid-Derived Second Messengers.- Detection of Myotubularin Phosphatases Activity on Phosphoinositides and.- Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts.- Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy.- Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy.- Phosphoinositide (PI) 3-Kinase Assays.- Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the