Lipid-Activated Nuclear Receptors

Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS.- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands.- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages.- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages.- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity.- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells.- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages.- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics.- Quantifying Cellular Cholesterol Efflux.- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection.- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency.- Methods to Study Monocyte and MacrophageTrafficking in Atherosclerosis Progression and Resolution.- Preparation of Frozen Liver Tissues for Integrated Omics Analysis.- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver.- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo.- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry.