Immune Homeostasis

Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays.- Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells.- Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes.- Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique.- Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation.- Analyses of Programmed Cell Death in Dendritic Cells.- Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity.- Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers.- Homeostatic Proliferation of Mature T Cells.- Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers.- Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis.- Molecular Measurement of T Cell Receptor Excision Circles.- Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis.- Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders.- Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP).- In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells.