Human Monoclonal Antibodies
Methods and Protocols
(Sprache: Englisch)
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human...
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Klappentext zu „Human Monoclonal Antibodies “
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.
Inhaltsverzeichnis zu „Human Monoclonal Antibodies “
Dedication...Preface...Table of Contents...Contributing Authors... 1. Human Monocolonal Antibodies: The Benefits of HumanizationHerman Waldmann 2. Cancer Immunotherapy: The Dawn of Antibody CocktailsIlaria Marrocco, Donatella Romaniello, and Yosef Yarden 3. IgM Natural Autoantibodies in Physiology and the Treatment of DiseaseMahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, and Moses Rodriguez 4. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future StrategiesMadhuri Bhandaru and Anand Rotte 5. An Efficient Method to generate Monoclonal Antibodies from Human B CellsJenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, and Patrick C. Wilson 6. Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot ArrayHiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, and Atsushi Muraguchi 7. Purification of Human Monoclonal antibodies and Their FragmentsNicole Ulmer, Sebastian Vigg, Thomas Müller-Späth, and Massimo Morbidelli 8. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral BloodAnna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, and Benjamin Frey 9. Humanization and Simultaneous Optimization of Monoclonal AntibodyT. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori 10. Antibody Fragments Humanization: Beginning with the End in MindNicolas Aubrey and Philippe Billiald 11. Antigen-Specific Human Monoclonal Antibodies from Transgenic MiceSusana Magadán Mompó and África González-Fernández 12. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab FrameworkJeremy D. King and John C. Williams 13. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen ReceptorsKeichiro Mihara, Tetsumi Yoshida, and Joyeeta Bhattacharyya 14. Rapid Chimerization of AntibodiesKoji Hashimoto, Kohei Kurosawa, Hidetaka Seo, and Kunihiro Ohta 15. Phage Display Technology
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for Human Monoclonal AntibodiesDal Ferro Marco, Rizzo Serena, Rizzo Emanuela, Marano Francesca, Luisis Immacoloata, Tarasiuk Olga, and Daniele Sblattero 16. Recombinant Antibody Selections by Combining Phage and Yeast DisplayFortunato Ferrara, Maria Felicia Soluri, and Daniele Sblattero 17. Epitope Mapping via Phage Display from Single Gene LibrariesViola Fühner, Philip Alexander Heine, Kilian Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira, Michael Hust, and Giulio Russo 18. A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display BiopanningAngela Chiew Wen Ch'ng, Azimah Ahmad, Zoltán Konthur, and Theam Soon Lim 19. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin Ivan Babic, Santosh Kesari, and Mark C. Glossy 20. A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan ReceptorsPatricia Blundell and Richard Pleass 21. A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal AntibodiesMichihiko Aoyama, Minoru Tada, and Akiko Ishii-Watabe 22. "BIClonals": Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian CellsDana Litvak-Greenfeld, Lilach Vaks, Stav Dror, Limor Nahary, and Itai Benhar 23. Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently-Transfected Mammalian CellsRacheli Birnboim-Perach, Yehudit Grinberg, Lilach Vaks, Limor Nahary, and Itai Benhar
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Bibliographische Angaben
- 2018, 2. Aufl., XIV, 483 Seiten, 59 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Michael Steinitz
- Verlag: Springer, Berlin
- ISBN-10: 149398957X
- ISBN-13: 9781493989577
- Erscheinungsdatum: 12.12.2018
Sprache:
Englisch
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