High Content Screening
A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery
(Sprache: Englisch)
This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures,...
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This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis zu „High Content Screening “
Part I: Reagents and Kits for Measuring Cells and their Phenotypes 1. Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays
Roy Edward
2. General Staining and Segmentation Procedures for High Content Imaging and Analysis
Kevin M Chambers, Bhaskar S Mandavilli, Nick J Dolman and Michael S Janes
3. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis
Bhaskar S Mandavilli, Robert J Aggeler, and Kevin Chambers
4. Cell-based High Content Analysis of Cell Proliferation and Apoptosis
Bhaskar S Mandavilli, Michelle Yann and Scott Clarke
5. Tools to Measure Autophagy using High Content Imaging and Analysis Nick J Dolman, Brent A. Samson, Kevin M. Chambers, Michael S Janes, and Bhaskar S Mandavilli
Part II: Selecting Microtiter Plates, Identify Imaging Artifacts, Rnai Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS
6. Guidelines for Microplate Selection in High Content Imaging
O.J. Trask
7. Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler
Mark-Anthony Bray and Anne E. Carpenter
8. High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening Steven A. Haney
9. Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays
Karol Kozak, Bernt Rinn, Oliver Leven, and M. Emmenlauer
10. Live-Cell High Content Screening in Drug Development
Milan Esner, Felix Meyenhofer, and Marc Bickle
11. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening
... mehr
Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O'Connell, Martyn Banks, and Andrea Weston
Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis
12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells
Verena Fetz, Roland H. Stauber, and Shirley K. Knauer
13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions
Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston
14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines
Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis
15. Single Cell and Population Level Analysis of HCA Data
David Novo, Kaya Ghosh, and Sean Burke
16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens
Judith Wardwell-Swanson and Yanhua Hu
Section IV: Phenotypic Models - Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos
17. High Content Screening of Mammalian Primary Cortical Neurons
Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon
18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications
Joshua A. Harrill
19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays
Oksana Sirenko and Evan F. Cromwell
20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates
David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, and Paul A. Johnston
21. An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro
Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, and Mark Uhlik
22. High-throughput Automated Chemical Screens in Zebrafish Manush Saydmohammed and Michael Tsang
Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O'Connell, Martyn Banks, and Andrea Weston
Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis
12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells
Verena Fetz, Roland H. Stauber, and Shirley K. Knauer
13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions
Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston
14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines
Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis
15. Single Cell and Population Level Analysis of HCA Data
David Novo, Kaya Ghosh, and Sean Burke
16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens
Judith Wardwell-Swanson and Yanhua Hu
Section IV: Phenotypic Models - Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos
17. High Content Screening of Mammalian Primary Cortical Neurons
Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon
18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications
Joshua A. Harrill
19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays
Oksana Sirenko and Evan F. Cromwell
20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates
David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, and Paul A. Johnston
21. An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro
Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, and Mark Uhlik
22. High-throughput Automated Chemical Screens in Zebrafish Manush Saydmohammed and Michael Tsang
... weniger
Bibliographische Angaben
- 2017, 2. Aufl., XIII, 397 Seiten, 71 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Paul A. Johnston, Oscar J. Trask
- Verlag: Springer, Berlin
- ISBN-10: 149397355X
- ISBN-13: 9781493973552
- Erscheinungsdatum: 30.10.2017
Sprache:
Englisch
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