Handbook of Single-Molecule Biophysics
(Sprache: Englisch)
During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. This book introduces these techniques and presents an extensive discussion of new insights obtained from them.
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During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. This book introduces these techniques and presents an extensive discussion of new insights obtained from them.
Klappentext zu „Handbook of Single-Molecule Biophysics “
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.
Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.
Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.
Inhaltsverzeichnis zu „Handbook of Single-Molecule Biophysics “
Chapter 1 - Single-molecule fluorescence tracking Ahmet Yildiz (UCSF) Chapter 2 - Single molecule studies in the membrane Gerhard Schuetz (Linz) Thomas Schmidt (Leiden) Chapter 3 - Single-molecule imaging in live cells Jie Xiao (Johns Hopkins) Chapter 4 - Superresolution imaging Xiaowei Zhuang (Harvard) Chapter 5 - Fluorescence Resonance Energy Transfer Achillefs Kapanidis (Oxford) Chapter 6 - Single-molecule enzymology Antoine van Oijen (Harvard) Chapter 7 - Rotary proteins Richard Berry (Oxford) Chapter 8 - Fluorescence Correlation Spectroscopy Petra Schwille (Dresden) Chapter 9 - Advanced Concepts of Fluorescence Fluctuation Spectroscopy Jörg Enderlein (Tübingen) Chapter 10 - Nucleic-acid analysis at the single-molecule level Amit Meller (Boston University) Chapter 11 - Nanopores: Geneneration and Single-Molecule Applications Stefan Howorka (UCL) Zuzanna Siwy (UC-Irvine) Chapter 12 - Optical trapping Michael Woodside (Alberta) Megan Valentine (UCSB) Chapter 13 - Magnetic tweezing Nynke Dekker (Delft) Chapter 14 - Protein/DNA unfolding Matthias Rief (Munich) Chapter 15 - Single molecule recognition Ziv Reich (Weizman) Peter Hinterdorfer (Linz) Chapter 16 - Nano-scale AFM imaging Simon Scheuring (Institut Curie) Daniel J. Müller (Dresden) Chapter 17 - High Speed AFM T. Ando (Kanazawa) Chapter 18 - Recognition imaging Yves Dufrene (Leuven) Peter Hinterdorfer (Linz) Chapter 19 - Atomic force microscopy of protein-protein interactions Vincent Moy (Miami) Chapter 20 - A New Approach to Analysis of Single MoleculeForce Measurements Evan Evans (Vancouver) Chapter 21 - Single Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation Cheng Zhu (Georgia Tech)
Bibliographische Angaben
- 2009, XXI, 626 Seiten, Masse: 17,8 x 25,4 cm, Gebunden, Englisch
- Herausgegeben: Peter Hinterdorfer, Antoine van Oijen
- Verlag: Springer, Berlin
- ISBN-10: 0387764968
- ISBN-13: 9780387764962
Sprache:
Englisch
Rezension zu „Handbook of Single-Molecule Biophysics “
From the reviews:"The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. ... Summing Up: Recommended. Graduate students, researchers, faculty, and professionals." (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)
Pressezitat
From the reviews: "The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. ... Summing Up: Recommended. Graduate students, researchers, faculty, and professionals." (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)
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