Fluorescence in situ Hybridization (FISH)
(Sprache: Englisch)
The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic...
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The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.
Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.
Klappentext zu „Fluorescence in situ Hybridization (FISH) “
Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.
The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.
Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.
Inhaltsverzeichnis zu „Fluorescence in situ Hybridization (FISH) “
Part I: Core Techniques1. Fluorescence in situ Hybridization (FISH), Basic Principles, and Methodology Elisa Garimberti and Sabrina Tosi2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibers Lauren S. Elcock and Joanna M. Bridger3. Detection of Nascent RNA Transcripts by Fluorescence in situ Hybridization Jill M. Brown and Veronica J. Buckle4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays Brenda M. Summersgill and Janet M. Shipley5. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections Irina Solovei6. Multiplex Fluorescence in situ Hybridization (M-FISH) Rhona Anderson7. Optical Mapping of Protein-DNA Complexes on Chromatin Fibers Beth Sullivan8. 3D-FISH on Cultured Cells Combined with Immunostaining Irina Solovei and Marion CremerPart II: Technical Advancements and Novel Adaptations9. The Comet-FISH Assay for the Analysis of DNA Damage and Repair Graciela Spivak10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes Laurent A. Bentolila11. LNA-FISH for Detection of microRNAs in Frozen Sections Asli N. Silahtaroglu12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISH Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Müller, Andriy Mokhir, Roland Krämer, Christoph Cremer, and Michael Hausmann14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation Daniela Moralli and Zoia L. Monaco15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin Cryosections Sheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding
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(MCB/m-band) Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo Hybridization Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. DirksPart III: Translational FISH: Applications for Human Genetics and Medicine18. Quality Control in FISH as Part of a Laboratory's Quality Management System Ros Hastings19. FlashFISH: "Same Day" Prenatal Diagnosis of Common Chromosomal Aneuploidies Sherry Ho and Mahesh A. Choolani20. FISH for Pre-Implantation Genetic Diagnosis Paul N. Scriven and Caroline Mackie Ogilvie21. PNA-FISH on Human Sperm Franck Pellestor, Cécile Monzo, and Samir Hamamah22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH) Agata Kowalska, Eva Bozsaky, and Peter F. Ambros24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material Soo-Yong Tan and Goran Mattsson25. Fluorescence in situ Hybridization on 3D Cultures of Tumor Cells Karen J. Meaburn26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease Monitoring Bruce PattersonPart IV: Protocols for Model Organisms27. Detection of Prokaryotic Cells with Fluorescence in situ Hybridization Katrin Zwirglmaier28. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast Harry Scherthan and Josef Loidl29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic
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Bibliographische Angaben
- XIV, 451 Seiten, 24 farbige Abbildungen, 24 Schwarz-Weiss-Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Joanna M. Bridger, Emanuela V. Volpi
- Verlag: Springer-Verlag GmbH
- ISBN-10: 1607617889
- ISBN-13: 9781607617884
- Erscheinungsdatum: 09.09.2010
Sprache:
Englisch
Rezension zu „Fluorescence in situ Hybridization (FISH) “
From the reviews:"Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. ... Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. ... I would suggest this is for microbiological libraries ... ." (Jonathan Porter, Microbiology Today, November, 2011)
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