Flow Cytometry and Cell Sorting

Preface - Leonard A. Herzenberg;

Part I Flow Cytometry: 1 Operation of a Flow Cytometer - Christoph Göttlinger, Birgit Mechtold and Andreas Radbruch;

Part II Immunofluorescence:
2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies - Werner Müller;
3 Immunofluorescence: Basic Considerations - Andreas Radbruch;
4 Multicolor Immunofluorescence Analysis - Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald;
5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells - Mario Assenmacher;
6 Cytometry and Sorting of Live Cells According to Their Secreted Products - Rudolf A. MANZ;
7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes - Alexander Scheffold;
8 Scatchard Analysis by Flow Cytometry - Robert F. Murphy;
9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry - Attila Trnok, Ursel Nöhrenberg, Hans-Jürgen Vollmer, Stephan Schuhmacher;

Part III DNA and Proliferation:
10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis - Friedrich J. Otto;
11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression - Werner Müller;
12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique - Manfred Kubbies;

Part IV Cellular Activation and Biochemistry:
13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium- Manfred Kubbies;
14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry - Attila Tarnok and Henning Ulrich;
15 Biochemical Parameters of Cell Function - Gregor Rothe and Günther Valet;
16 The FACS-Gal Assay - Steffen Jung;
17 Ligand Acidification by Nonadherent Cells - Robert F. Murphy; Part V Cell Sorting:
18 Powerful Preselection - Charlotte Esser;
19 Magnetic Selection of Transfected Cells - Gregor Siebenkotten and Ute Behrens-Jung;
20 High