Epitope Mapping Protocols
(Sprache: Englisch)
This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part...
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This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.
Inhaltsverzeichnis zu „Epitope Mapping Protocols “
Preface...Table of Contents...Contributing Authors... 1. An Introduction to Epitope MappingJohan Nilvebrant and Johan Rockberg Part I Whole Antigen Methods 2. Epitope Mapping of Antibody-Antigen Interactions with X-Ray CrystallographyMoeko Toride and Cory L. Brooks 3. Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical ConsiderationsLuca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, and Luca Varani 4. Label-Free Cell-Based Assay for Characterization of Biomolecules and ReceptorsDiluka Peiris and Lars M. Jørgensen 5. Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-LinkersThomas Huber and Thomas P. Sakmar 6. Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep SequencingTariq Ahmad Najar, Shruti Khare, and Raghavan Varadarajan 7. Epitope Mapping Using Yeast Display and Next Generation SequencingThomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton, Dilduz Telman, Lora Zhao, Wai Ling Cheung, Jan Berka, Wenwu Zhai, Pavel Strop, Jaume Pons, Arvind Rajpal, and Javier Chaparro-Riggers Part II Protein Domain and Fragment-Based Methods 8. B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent AssayThiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, and Michael W. Heuzenroeder 9. Array-In-Well Epitope Mapping of Phage-Displayed AntibodiesUrpo Lamminmäki, Gaurav Batra, and Petri Saviranta 10. Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment LibrariesAnna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg 11. Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded DomainsAnna-Luisa Volk and Johan Rockberg Part III Real-Time Based Methods 12. Kinetic Analysis and Epitope Binning Using Surface Plasmon ResonanceJohan Nilvebrant 13. Epitope
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Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer InterferometryLisette Bronswijk-Deddens Part IV Antibody Specificity and T-Cell Prediction 14. Antibody Specificity Profiling Using Protein MicroarraysPedro Ramos-López, José Irizarry, Ignacio Pino, and Seth Blackshaw 15. High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target BindingRonald Sjöberg, Eni Andersson, Cecilia Hellström, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson, and Burcu Ayoglu 16. Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope MappingBurcu Ayoglu, Peter Nilsson, and Jochen M. Schwenk 17. Antibody Biomarker Discovery Via In Vitro Evolution of PeptidesJohn T. Ballew, Jack R. Reifert, and Patrick S. Daugherty 18. Bioinformatics Tools for the Prediction of T Cell EpitopesMassimo Andreatta and Morten Nielsen
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Bibliographische Angaben
- 2018, 3. Aufl., XI, 284 Seiten, 38 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Johan Rockberg, Johan Nilvebrant
- Verlag: Springer, Berlin
- ISBN-10: 149397839X
- ISBN-13: 9781493978397
- Erscheinungsdatum: 01.05.2018
Sprache:
Englisch
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