Citrus Tristeza Virus
Methods and Protocols
(Sprache: Englisch)
This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific...
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This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.
Inhaltsverzeichnis zu „Citrus Tristeza Virus “
1. A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) MethodologiesMoshe Bar-Joseph
2. A Brief Historical Account of the Family Closteroviridae
Giovanni P. Martelli
3. Phenotyping Biological Properties of CTV Isolates
Marcella Russo and Antonino F. Catara
4. CTV Vectors and Interactions with the Virus and Host Plants
Raymond Yokomi
5. Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues
Mariano Cambra, Eduardo Vidal, Carmen Martínez, and Edson Bertolini 6. Detection of Citrus Tristeza Virus and Co-Infecting Viroids
Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, and Francesco Di Serio
7. Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP
Elisavet K. Chatzivassiliou and Grazia Licciardello
8. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus
Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, and Raymond Yokomi
9. Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device
Giuseppe Scuderi, Antonino Catara, and Grazia Licciardello
10. Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Ampli¿cation (RT-LAMP) Assay
Dilip Kumar Ghosh, Ashish Warghane, and Kajal Kumar Biswas
11. Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome
Munir Mawassi, Sabrina Haviv, and Ludmila Meslenin
12. Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus
Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, and Hans Jacob Maree
13. Analysis of Genotype Composition of Citrus
... mehr
Tristeza Virus Populations Using Illumina Miseq Technology
David A. Read and Gerhard Pietersen
14. Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction
Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio, and Ricardo Flores
15. Proteomic Response of Host Plants to Citrus Tristeza Virus
Milena Santos Dória and Carlos Priminho Pirovani
16. Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment
Yosvanis Acanda, Chunxia Wang, and Amit Levy
17. Methods for Producing Transgenic Plants Resistant to CTV
Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña
David A. Read and Gerhard Pietersen
14. Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction
Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio, and Ricardo Flores
15. Proteomic Response of Host Plants to Citrus Tristeza Virus
Milena Santos Dória and Carlos Priminho Pirovani
16. Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment
Yosvanis Acanda, Chunxia Wang, and Amit Levy
17. Methods for Producing Transgenic Plants Resistant to CTV
Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña
... weniger
Bibliographische Angaben
- 2019, 1st ed. 2019, X, 247 Seiten, 36 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Antonino F. Catara, Moshe Bar-Joseph, Grazia Licciardello
- Verlag: Springer, Berlin
- ISBN-10: 149399557X
- ISBN-13: 9781493995578
- Erscheinungsdatum: 25.06.2019
Sprache:
Englisch
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