Advanced Protocols for Animal Transgenesis

Karen S. Canady: Patent and licensing issues in transgenic technology.- Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.- Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.- Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.- Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.- Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.- Séverine Ménoret, Séverine Remy, et al : Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.- Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.- Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.- Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.- Aron Geurts, Lajos Mates, Darius Balciunas: Vertebrate Transgenesis by Transposition.- Karen M. Chapman et al.: Rat Spermatogonial Stem Cell Mediated Gene Transfer.- Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.- Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.- Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.- Marina Gertsenstein: Tetraploid Complementation Assay.- Elizabeth Williams et al.: Combining ES cells with Embryos.- Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.- Ping Li et al.: Rat Embryonic Stem Cell Derivation and Propagation.- Han Li et al.: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.- Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.- Karen Brennan: Colony Management.- B. Pintado and J Hourcade: Cryopreservation.- ShirleyPease: