X-Ray Crystallography of Biomacromolecules (PDF)
A Practical Guide
(Sprache: Englisch)
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. Clearly structured, Part A covers relevant...
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Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. Clearly structured, Part A covers relevant experimental techniques for crystallization, generation of X-rays and data collection, the principles of X-ray diffraction, solutions for the phase problem and further steps for structure determination, time-resolved X-ray crystallography and structural genomics. Part B includes case studies for different stages in determining X-ray crystal structure, such as diffraction data evaluation, MIRAS and MAD techniques, molecular replacement, phase improvement by electron density averaging, non-crystallographic symmetries and model building.
With its coverage of the theory, practice and latest achievements in x-ray crystallography, this book will benefit any researcher in structural biology.
Albrecht Messerschmidt is head of a research group at Max-Planck-Institute for Biochemistry in Martinsried, Germany, department of proteomics and signal transduction headed by Matthias Mann. He gained his PhD in Crystallography at the Humboldt University in Berlin under Bill Kleber. After working on the X-ray crystallography of small biological molecules he moved to the field of X-ray crystallography of biomacromolecules, working in the department of structural research under Nobel laureate Robert Huber. He gained his lecturing qualification in the field of physical biochemistry at the faculty of biology of the University of Constance in 1996, where he was appointed extraordinary professor in 2002.
The author has published great number of scientific papers and is one of the editors of the three volume Handbook of Metalloproteins, published by Wiley.
With its coverage of the theory, practice and latest achievements in x-ray crystallography, this book will benefit any researcher in structural biology.
Albrecht Messerschmidt is head of a research group at Max-Planck-Institute for Biochemistry in Martinsried, Germany, department of proteomics and signal transduction headed by Matthias Mann. He gained his PhD in Crystallography at the Humboldt University in Berlin under Bill Kleber. After working on the X-ray crystallography of small biological molecules he moved to the field of X-ray crystallography of biomacromolecules, working in the department of structural research under Nobel laureate Robert Huber. He gained his lecturing qualification in the field of physical biochemistry at the faculty of biology of the University of Constance in 1996, where he was appointed extraordinary professor in 2002.
The author has published great number of scientific papers and is one of the editors of the three volume Handbook of Metalloproteins, published by Wiley.
Inhaltsverzeichnis zu „X-Ray Crystallography of Biomacromolecules (PDF)“
PART A THEORETICAL BASIS AND EXPERIMENTAL TECHNIQUES Introduction Techniques Principles of X-ray Diffraction by a Crystal Methods for Solving the Phase Problem Model Building and Refinement Time-resolved X-ray Crystallography PART B CASE STUDIES FOR DIFFERENT KINDS OF X-RAY CRYSTAL STRUCTURE DETERMINATION OF BIOMACROMOLECULES Space Group Determination and Data Reduction Phase Determination by MIRAS Technique Phase Determination by MAD Technique Phase Determination by Molecular Replacement Difference Fourier Technique Map Improvement by NCS Averaging and other Methods Automated Model Building Structure Validation and PDB-Submission
Autoren-Porträt von Albrecht Messerschmidt
Professor Dr. Albrecht Messerschmidt is group leader at the Max Planck Institute for Biochemistry, Marinsried, Germany.His main reserach interest is in the determination of X-ray structures of metalloproteins and -enzymes, PLP-dependent enzymes, CoA-transferases and other proteins. His group is part of the department of X-ray structure determination of proteins headed by nobel laureate Robert Huber.
Bibliographische Angaben
- Autor: Albrecht Messerschmidt
- 2007, 1. Auflage, 305 Seiten, Englisch
- Verlag: Wiley-VCH GmbH
- ISBN-10: 3527609679
- ISBN-13: 9783527609673
- Erscheinungsdatum: 27.02.2007
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