Molecular Biology Problem Solver (PDF)
A Laboratory Guide
(Sprache: Englisch)
Most research in the life sciences involves a core set of
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial...
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial...
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Most research in the life sciences involves a core set of
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial technical
support groups, especially regarding problems.
Molecular Biology Problem Solver: A Laboratory
Guide asks the reader to consider crucial questions, such
as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful
application of a technique?
* Are you familiar with the limitations of a given
technique?
* When should common procedural rules of thumb not be
applied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and
laboratory practices, with the aim of offering a firm understanding
of how techniques and procedures work, as well as how to avoid
problems. Some major issues explored by the book's expert
contributors include:
* Working safely with biological samples and radioactive
materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial technical
support groups, especially regarding problems.
Molecular Biology Problem Solver: A Laboratory
Guide asks the reader to consider crucial questions, such
as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful
application of a technique?
* Are you familiar with the limitations of a given
technique?
* When should common procedural rules of thumb not be
applied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and
laboratory practices, with the aim of offering a firm understanding
of how techniques and procedures work, as well as how to avoid
problems. Some major issues explored by the book's expert
contributors include:
* Working safely with biological samples and radioactive
materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment
Inhaltsverzeichnis zu „Molecular Biology Problem Solver (PDF)“
Preface. Contributors. Preparing for Success in the Laboratory (P. Franciskovich). Getting What You Need from a Supplier (T. Tyre & G. Krueger). The Preparation of Buffers and Other Solutions: A Chemist's Perspective (E. Pfannkoch). How to Properly Use and Maintain Laboratory Equipment (T. Troutman, et al.). Working Safely with Biological Samples (C. Haidaris & E. Brownlow). Working Safely with Radioactive Materials (W. Volny). DNA Purification (S. Herzer). RNA Purification (L. Martin, et al.). Restriction Endonucleases (D. Robinson, et al.). Nucleotides, Oligonucleotides, and Polynucleotides (A. Gerstein). PCR (K. Aoyagi). Electrophoresis (M. Booz). Western Blotting (P. Riis). Nucleic Acid Hybridization (S. Herzer & D. Englert). E. coli Expression Systems (P. Bell). Eukaryotic Expression (J. Trill, et al.). Index.
Autoren-Porträt von Alan S. Gerstein
ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.
Bibliographische Angaben
- Autor: Alan S. Gerstein
- 2004, 1. Auflage, 596 Seiten, Englisch
- Verlag: John Wiley & Sons
- ISBN-10: 0471461032
- ISBN-13: 9780471461036
- Erscheinungsdatum: 24.03.2004
Abhängig von Bildschirmgrösse und eingestellter Schriftgrösse kann die Seitenzahl auf Ihrem Lesegerät variieren.
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- Grösse: 2.08 MB
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Sprache:
Englisch
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