Zebrafish Protocols for Neurobehavioral Research
(Sprache: Englisch)
Zebrafish play an integral role in biomedical research, enabling scientists to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy. Written by experts, this volume sets out protocols for both larval and adult models.
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Zebrafish play an integral role in biomedical research, enabling scientists to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy. Written by experts, this volume sets out protocols for both larval and adult models.
Klappentext zu „Zebrafish Protocols for Neurobehavioral Research “
Zebrafish (Danio rerio) play an integral role in biomedical research, enabling researchers to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy. That, along with their low cost, easy manipulation, short reproductive cycles, and physiological homology to humans, has made zebrafish a vital model organism for neuroscience research. Zebrafish Protocols for Neurobehavioral Research addresses protocols for both larval and adult models, written by the leading experts in the field of zebrafish research. Part I of this book takes advantage of the high-throughput nature of larval models to offer protocols for research requiring high output, easily manipulated screens. The second half of the book focuses on the robust and sophisticated behaviors of adult zebrafish, suitable for the neurophenotyping of complex traits and multi-domain disorders. Importantly, these models complement each other, working together to provide researchers with valuable insights into neurobiology of normal and pathological behavior. Thorough and cutting-edge, this volume is a useful, authoritative reference guide that should hold a coveted spot in zebrafish laboratories across the globe.
Inhaltsverzeichnis zu „Zebrafish Protocols for Neurobehavioral Research “
Measuring Larval Zebrafish Behavior: Locomotion, Thigmotaxis, and Startle.- The Light-Dark Preference Test for Larval Zebrafish.- Assessment of Thigmotaxis in Larval Zebrafish.- Zebrafish and Drug Development: A Behavioral Assay System for Probing Nicotine Function in Larval Zebrafish.- Olfactory Evoked Activity Assay for Larval Zebrafish.- Qualitative and Quantitative Measurements of the Optokinetic Response in Larval Zebrafish.- Automated Imaging of Visual Recognition Memory in Larval Zebrafish.- Automated Conditioning in Larval Zebrafish.- A Method to Quantify Basal and Stress-Induced Cortisol Response in Larval Zebrafish.- A Method for Sorting Zebrafish on the Exploratory-Bold Behavioral Axis.- Nocifensive Behavior in Adult and Larval Zebrafish.- Use of a Split Depth and Visual Cliff Apparatus to Measure the Diving Response in Zebrafish.- Adapting the Open Field Test to Assess Anxiety-Related Behavior in Zebrafish.- Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test.- A High Throughput and Inexpensive Assay for Anxiety-Related Behaviors in the Zebrafish, Based on Place Preference and Latency to Feed.- Automated Tracking of Zebrafish Shoals and the Analysis of Shoaling Behavior.- Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests.- Measuring Effects of Psychostimulants on Egocentric Spatial Learning and Memory in Adult Zebrafish.- Cued Fear Conditioning in Zebrafish (Danio rerio).- Assessing Learning and Memory Through the Active Avoidance Paradigm.- Assessing Habituation Phenotypes in Adult Zebrafish: Intra- and Inter-Trial Habituation in the Novel Tank Test.- Assessing Startle Responses and Their Habituation in Adult Zebrafish.- QTL Mapping Using Behavioral Traits in the Adult Zebrafish.- Assessing Epilepsy-Related Behavioral Phenotypes in Adult Zebrafish.- Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish.- Western Blotting Assay for Egr-1
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Immediate Early Gene in Brain Tissue of Zebrafish Applied to Neuroethological Study.- Utilizing the Zebrafish Neurophenome Project (ZNP) Database for Analyses of Complex Neurophenotypes in Zebrafish Models.
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Bibliographische Angaben
- 2016, Softcover reprint of the original 1st ed. 2012, XVII, 357 Seiten, 22 farbige Abbildungen, Masse: 15,9 x 23,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Allan V. Kalueff, Adam Michael Stewart
- Verlag: Springer, Berlin
- ISBN-10: 1493960024
- ISBN-13: 9781493960026
Sprache:
Englisch
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