Virus-Host Interactions

A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions.- Analysis of Protein-Protein Interactions Using LUMIER Assay.- Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation.- Discovery of Host-Viral Protein Complexes During Infection.- Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi.- High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine.- Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape.- Computational Analysis of Virus-Host Interactomes.- Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins.- Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH).- Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes.- Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line.- Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs.- Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry.- High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time.- Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells.- 3D Tissue Model for Herpes Simplex Virus-1Infections.- In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI).- Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry.- Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells.- Assessment of Natural Killer Cell Responses to Human Cytomegalovirus