Vascular Disease

Genetics in Vascular Disease.- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis.- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis.- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes.- Isolation of Genes Expressed in Vasular Tissue.- Efficient Extraction of RNA from Vascular Tissue.- Preparation of cDNA Libraries from Vascular Cells.- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells.- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR.- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques.- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells.- Identification of Novel Protein Kinases in Vascular Cells.- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells.- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes.- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells.- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells.- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells.- Molecular Analysis of MRNA Expression in Vascular Cells.- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis.- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases.- Localization of Gene Expression inHuman Atherosclerotic Lesions by In SituHybridization.- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue.- Molecular Methods to Study Apoptosis and Phenotypic Changes in

"...The chapters are clearly written so that complex techniques are reduced to their individual components...this book will be a major asset to a molecular biology laboratory involved in vascular research."-Atherosclerosis

"This collection of very detailed molecular biology protocols was compiled...The first five parts present protocols that are designed to identity and characterize genes in cardiovascular disease, including methods for detecting specific gene mutation, identifying new genes, characterizing the levels and regulation of expression of the new genes, and apoptotic methods. The final two parts contain in vivo and in culture gene transfer protocols used in cardiovascular disease and protocols for clinical gene therapy....an excellent reference for molecular biologists in the field of vascular disease." - Quarterly Review of Biology

"...the authors of the individual chapters have made every effort to relate their particular techniques to the study of vascular tissues....the editor has assembled a broad base of well established authors to describe their protocols....this is a good book....this book may prove worthwhile in helping to ease the load on the typical physician-scientist trying to care for patients and at the same time direct his or her laboratory research program."- Texas Heart Institute Journal

"The book is the result of the contribution of distinguished experts in the field. Each chapter of the book is introduced by a theoretical summary of the goal of the technique with excellent diagrams followed by the material used, the basic procedures and the methods. Critical notes and references end each chapter. This important publication directed to molecular biologists covers the latest aspects of molecular procedures for the study of cardiovascular diseases and confirms, if necessary, the irreversible revolution in cardiovascular research."- Acta Cardiologica