Synthetic Protein Switches

Synthetic Protein Switches: Theoretical and Experimental Considerations.- Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange.- Construction of Protein Switches by Domain Insertion and Directed Evolution.- Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen.- Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors.- Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment.- Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs.- Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases.- Quantitative and Dynamic Imaging of ATM Kinase Activity.- Creation of Antigen-Dependent beta-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains.- Protein and Protease Sensing by Allosteric Derepression.- DNA-Specific Biosensors Based on Intramolecular beta-Lactamase-Inhibitor Complex Formation.- Engineering and Characterizing Synthetic Protease Sensors and Switches.- Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV.- Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light.- Light-Regulated Protein Kinases Based on the CRY2-CIB1 System.- Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels.- Primer-Aided Truncation for the Creation of Hybrid Proteins.- Engineering Small Molecule Responsive Split-Protein Kinases.- Directed Evolution Methods to Rewire Signaling Networks.