Structural Proteomics

Protein Target Selection, Bioinformatic Approaches, and Data Management.- Target Selection for Structural Genomics: An Overview.- A General Target Selection Method for Crystallographic Proteomics.- Target Selection: Triage in the Structural Genomics Battlefield.- Data Management in Structural Genomics: An Overview.- Data Deposition and Annotation at the Worldwide Protein Data Bank.- Prediction of Protein Disorder.- Protein Domain Prediction.- Protein Structure Modeling with MODELLER.- Protein Production.- High Throughput Cloning with Restriction Enzymes.- Automated Recombinant Protein Expression Screening in Escherichia coli.- From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of the Target Proteins with Randomized N-Termini.- Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli.- A High Throughput Platform for Eukaryotic Genes.- High Throughput Production of Recombinant Human Proteins for Crystallography.- Assembly of Protein Complexes by Coexpression in Prokaryotic and Eukaryotic Hosts: an Overview.- Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy.- A Medium or High Throughput Protein Refolding Assay.- Structural Proteomics of Membrane Proteins: a Survey of Published Techniques and Design of a Rational High Throughput Strategy.- Biophysical and Functional Characterization of Proteins.- Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light Scattering.- High Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram ScaleHigh Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale.- High Throughput Screening of Purified Proteins for Enzymatic Activity.-Structural Characterization of Proteins.- Strategies for Improving Crystallization Success Rates.- Protein Crystallization in Restricted Geometry: Advancing Old Ideas for Modern Times in Structural

The objective of this volume of Methods in Molecular Biology on Structural Proteomics: High-throughput methods is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This is the first book of protocols to cover techniques in a new and emerging field. The volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. The readers are encouraged to access further details on the methodologies in on-line resources and in the cited literature. We emphasize that most methods are as amenable to small laboratories as to large consortia, and do not require major investments in facilities. It is hoped that this volume will help smaller laboratories establish some of the high-throughput techniques.

From the reviews:



"This book compiles an impressive array of contributions from a variety of contributors ... usually in a format that is accessible to any jobbing structural biologist, or those other molecular biologists with an linking to press their molecular targets into crystal screens or NMR tubes. ... In sum, this is a book it is useful to have to hand ... ." (Paul C. Driscoll, ChemBioChem, Issue 9, August, 2008)

"This book ... details some of the `tricks of the trade' involved in the field of structural genomics. ... This will be a useful text for laboratories already involved in fields covered in the book who want to increase the number of projects in the lab or for those who want to expand into neighbouring fields." (Darren Thompson, Microbiology Today, November, 2008)