Small Molecule Microarrays
Methods and Protocols
(Sprache: Englisch)
Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a...
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Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heights
Inhaltsverzeichnis zu „Small Molecule Microarrays “
The Expanding World of Small Molecule Microarrays.- Novel Substrates for Microarrays.- Fabrication of Bio-function-preserved Saccharide Microarray Chips with Cyanuric Chloride as a Rotatable Linker.- Fabrication of Carbohydrate Microarrays by Boronate Formation.- Clickable Polymeric Coating for Glycan Microarrays.- A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction between Tetrazine and Trans-Cyclooctene.- Label-Free Sensing on Microarrays.- Optical Microscopy for Detecting Binding on Small Molecule Microarrays.- Array-on-array Strategy For Activity-based Enzyme Profiling.- Protein-Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays.- Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays.- Profiling Phosphopeptide-binding Domain Recognition Specificity using Peptide Microarrays.- Validation Procedure for Multiplex Antibiotic Immunoassays UsingFlow-based Chemiluminescence Microarrays.- Large-scale Interaction Profiling of Protein Domains through Proteomic Peptide-PhageDisplay using Custom Peptidomes.- Synthetic Glycan Microarrays.- Screening Mammalian Cells on a Hydrogel- Functionalized Small Molecule Microarray.Bibliographische Angaben
- 2018, 2. Aufl., XI, 260 Seiten, 60 farbige Abbildungen, Masse: 17,8 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Mahesh Uttamchandani, Shao Q. Yao
- Verlag: Springer, Berlin
- ISBN-10: 1493982427
- ISBN-13: 9781493982424
Sprache:
Englisch
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