SARS- and Other Coronaviruses
Laboratory Protocols
(Sprache: Englisch)
In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through 23 state-of-the-art, widely applicable protocols with minute detail....
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Produktinformationen zu „SARS- and Other Coronaviruses “
In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through 23 state-of-the-art, widely applicable protocols with minute detail. Comprehensive and cutting-edge, the book serves as an ideal guide for all virologists and especially for those working with coronaviruses. Written by international experts, this book is relevant to a wide array of professions.
Klappentext zu „SARS- and Other Coronaviruses “
In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors.Comprehensive and cutting-edge, SARS- and Other Coronaviruses Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.
In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.ocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and c
Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.ocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and c
Inhaltsverzeichnis zu „SARS- and Other Coronaviruses “
Detection and Discovery of Coronaviruses.- A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses.- Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions.- Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m.- RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot.- Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains.- Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions.- Detection of New Viruses by VIDISCA.- Isolation, Growth, Titration, and Purification of Coronaviruses.- Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay.- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration.- Isolation and Propagation of Coronaviruses inEmbryonated Eggs.- Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen.- Structure of Covs Analyzed by Electron Microscopy.- Purification and Electron Cryomicroscopy of Coronavirus Particles.- Expression of Coronavirus Proteins and Crystallization.- Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization.- Raising Antibodies to Coronavirus Proteins.- Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus.- Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus.- Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins.- Manipulating the Genomes of Coronaviruses.- Manipulation of the Coronavirus GenomeUsing Targeted RNA Recombination with Interspecies Chimeric Coronaviruses.- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral
... mehr
Replicon RNAs.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes.- Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome.- Identifying Coronavirus Receptors.- Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes.- Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins.
... weniger
Bibliographische Angaben
- 2010, XV, 326 Seiten, Masse: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben: Dave Cavanagh
- Verlag: Springer, Berlin
- ISBN-10: 1617378151
- ISBN-13: 9781617378157
Sprache:
Englisch
Pressezitat
From the reviews: "The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening consequences in humans. ... this book describes the laboratory techniques used to study them. ... Scientists and students working in virology laboratories and, specifically, laboratories working with coronaviruses are the intended audience. ... each chapter contains a copious amount of detailed information as well as specific notes with technical advice. This is a valuable resource for scientists working with coronaviruses." (Rebecca T. Horvat, Doody's Review Service, November, 2008)
"This is volume 454 in the series Methods in Molecular Biology. ... if you work on coronaviruses, or want to enter the field, this is a must-have book at an affordable price." (Stuart Siddell, Microbiology Today, February, 2009)
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