Ribozymes

INTRODUCTION The chemical principles of RNA catalysis NATURALLY OCCURRING RIBOZYMES Biological roles of self-cleaving ribozymes Chemical mechanisms of the nucleolytic ribozymes The glmS ribozyme and its multifunctional coenzyme glucosamine-6-phosphate The lariat capping ribozyme Self-splicing group II introns The Spliceosome: an RNA-Protein ribozyme derived from ancient mobile genetic elements The ribosome and protein synthesis The RNase P ribozyme Ribozyme Discovery in Bacteria Small self-cleaving ribozymes in the genomes of vertebrates ENGINEERED RIBOZYMES Phosphoryl transfer ribozymes RNA replication and the RNA polymerase ribozyme Maintenance of genetic information in the first ribocell Ribozyme-catalyzed RNA recombination Engineering of hairpin ribozymes for RNA processing reactions Engineering of the Neurospora Varkud Satellite ribozyme for cleavage of non-natural stem-loop substrates Chemical modifications in natural and engineered ribozymes Ribozymes for regulation of gene expression Development of flexizyme aminoacylation ribozymes and their applications In vitro selected (deoxy)ribozymes that catalyze carbon-carbon-bond formation Nucleic acid catalyzed RNA ligation and labeling DNAZYMES The chemical repertoire of DNA enzymes Light-utilizing DNAzymes Applications of DNAzymes in computing and nanotechnology. RIBOZYMES AS TOOLS IN DIAGNOSTICS AND THERAPY Optimization of antiviral ribozymes DNAzymes as Biosensors Compartmentalization-based technologies for in vitro selection and evolution of ribozymes and light-up RNA aptamers TOOLS AND METHODS TO STUDY RIBOZYMES Elucidation of ribozyme mechanisms at the example of the Pistol ribozyme Strategies for crystallization of natural ribozymes NMR spectroscopic investigation of ribozymes Studying Ribozymes with Electron Paramagnetic Resonance Spectroscopy Computational modeling methods for 3D structure prediction of ribozymes

Sabine Müller is Full Professor for Biochemistry/Bioorganic Chemistry at Greifswald University (Germany). Having obtained her academic degrees from Humboldt University Berlin, she spent one and a half years as a postdoc at the MRC in Cambridge (UK) and then took up a group leader position again at Humboldt University. After finishing her Habilitation in 2003, she moved to the Ruhr University Bochum, where she was a Professor for Bioorganic Chemistry, before taking up her present appointment at Greifswald in 2006. Professor Müller has received several scientific awards, including a Heisenbergstipendium of the Deutsche Forschungsgemeinschaft (DFG). She is a member of the Leibniz-Societät der Wissenschaften zu Berlin and of AcademiaNet. Benoît Masquida is a research director at Centre National de la Recherche Scientifique. After graduating from Université Louis Pasteur in Strasbourg (France) working on the rules governing RNA folding, he spent one year at Yale University concentrating on ribosomal RNA structural motifs. After coming back to Strasbourg, he was appointed as a CNRS Research Scientist. He was promoted to Research Director in 2016, acknowledging his important contributions in the RNA structural biology field. Wade Winkler is an Associate Professor of Cell Biology and Molecular Genetics at the University of Maryland (USA). He acquired a graduate degree from The Ohio State University before spending three years as a postdoctoral researcher at Yale University. In 2004, he began his independent scientific career at The University of Texas Southwestern Medical Center in the Department of Biochemistry, and relocated his laboratory to the University of Maryland in 2011. Through the years, Professor Winkler has authored multiple influential publications on the different types of regulatory RNAs in bacteria.