Proteomics Sample Preparation
(Sprache: Englisch)
This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the...
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Klappentext zu „Proteomics Sample Preparation “
This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Dieser Laborleitfaden beschreibt die Praxis zur Analyse von Eiweiverbindungen und schliet damit eine echte Lcke in der Literatur. Er erlutert Vorbereitung, Durchfhrung und Auswertungsmethoden in Abhngigkeit von den Informationen, die eine Laboruntersuchung erbringen soll.
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This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
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This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Inhaltsverzeichnis zu „Proteomics Sample Preparation “
- Introduction- General Aspects
- Proteomics: A Philosophical Perspective
- MASS SPECTROMETRY
- ESI LC MS
- MALDI MS
- FT-ICR MS
- Labelling for MS
- ELECTROPHORESIS
1 -2 DGE
- Native Electrophoresis
- Free Flow Electrophoresis
- Capillary Electrophoresis
- OPTICAL METHODS
- Arrays
- Visual Proteomics
- Optical Proteomics on Cell Arrays
- Laser Capture Microdissection
- FACS
- CHROMATOGRAPHY
- HPLC
- Thin-Layer Chromatography
- STRUCTURAL PROTEOMICS
- NMR
- Crystallography
- INTERACTION ANALYSIS
- Tandem Affinity Purification (TAP) Technology
- Membrane Proteomics
- POST TRANSITIONAL MODIFICATIONS
- Phosphoanalysis
- Glycoanalysis
- SPECIES DEPENDENT PROTEOMICS
- Plant Proteomics
- Bacterial Proteomics
- Yeast & Fungi
- HUMAN PROTEOME
- Clinical Proteomics
- Stem Cell Proteomics
- Organelle Proteomics
- BIOINFORMATICS
- Bioinformatics Support for MS Quality Control
- Use of Physiochemical Properties in P&P Identification
Autoren-Porträt
Jg von Hagen is head of proteome research products development at Merck KGaA in Darmstadt (Germany). Having studied biotechnology in Giessen and Darmstadt, he received his academic degree with an award-winning thesis in 2001. Dr. von Hagen has more than 15 years of practical expertise in biotechnology, especially in molecular biology and proteomics methods for clinical applications.
Bibliographische Angaben
- 2008, XXXII, 453 Seiten, 12 farbige Abbildungen, 82 Schwarz-Weiss-Abbildungen, Masse: 17,7 x 24,6 cm, Gebunden, Englisch
- Herausgegeben: Jörg von Hagen
- Verlag: Wiley-VCH
- ISBN-10: 3527317961
- ISBN-13: 9783527317967
- Erscheinungsdatum: 26.03.2008
Sprache:
Englisch
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