Protein Structure, Stability, and Interactions

1 Microcalorimetry of Proteins and Their Complexes Peter L. Privalov

2 Determining the Conformational Stability of a Protein Using Urea Denaturation Curves Kevin L. Shaw, J. Martin Scholtz, C. Nick Pace, and G. R. Grimsley

3 Defining the Stability of Multimeric Proteins John W. Shriver and Stephen P. Edmondson

4 Protein-Protein and Ligand-Protein Interactions Studied by Analytical Ultracentrifugation Walter Stafford

5 Monitoring Molecular Interactions by NMR James M. Lipchock and J. Patrick Loria

6 Ligand Binding Interactions and Stability John W. Shriver and Stephen P. Edmondson

7 A Method for Direct Measurement of Protein Stability in Vivo Zoya Ignatova and Lila Gierasch

8 Quantifying the Roles of Water and Solutes (Denaturants, Osmolytes, and Hofmeister Salts) in Protein and Model Processes Using the Solute Partitioning Model Laurel M. Pegram and M. Thomas Record, Jr.

9 Molecular Crowding and Solvation: Direct and Indirect Impact on Protein Reactions Jörg Rösgen

10 Defining the Role of Salt Bridges in Protein Stability Ilian Jelesarov and Andrey Karshikoff

11 Protein Stabilization by the Rational Design of Surface Charge-Charge Interactions Katrina L. Schweiker and George I. Makhatakze

12 Analysis of Native State Protein Conformational Flexibility by Hydrogen Exchange Griselda Hernandez and David M. LeMaster

13 Single-Molecule Fluorescence Studies of Protein Folding G. Ulrich Nienhaus

14 Experimental Characterization of the Denatured State Ensemble of Proteins Jae-Hyun Cho and Dan Raleigh

From the reviews:



"Protein Structure, Stability, and Interactions covers a thorough list of biophysical methods as applied to proteins and their environments in vivo, in vitro, and sometimes in silico. ... The book begins with a tutorial on microcalorimetry by Privalov, followed by a chapter on useful protocols for urea denaturation curves ... . As a whole, this book would work as a text for an advanced course in biophysical methods or as a reference book for a biophysics laboratory." (Christopher Bystroff, Journal of the American Chemical Society, Vol. 131 (23), 2009)