Plant tissue culture

This textbook on "Plant Tissue culture"has been specially designed to need of researchers post and graduate students of university and college s of the country.this book emboded 06 chapters on the Momordi a dioica Roxb ex Wills. ucurbitaecae family,it's used as vegetables as well as medicinaly by rural and urban people.using second chapter review of literature. Third chapter material and methods applying different explant such as leaf, node,internode,tendril and shoot tip of momordica dioica by using MS Medium suplimented with various concentration and combination of auxin and cytokinin.forth chapter result and discussion,explant,media and hormonal evaluation by using suitable table and obtained results photograph on different media, various explant and invitro shoot regeration from callus culture,direct organogenesis,effect of BAP and Kn on direct shoot on nodal and shoot tip explant proliferation and elongation of multiple shoot,effect of auxin, rooting of excised shoot on halfstrength ms medium, hardening,pot transfer ,last using reference are cited the author is hope to present book shall prove more useful to researchers,M SC,Bsc.studsof botany and biotechnology.