Plant Signal Transduction

Multiple Dimensions in Plant Signal Transduction: An Overview.- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions.- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis.- Accurate Real-time Reverse Transcription Quantitative PCR.- Probing Spatio-temporal Intracellular Calcium Variations in Plants.- Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy.- Imaging of Reactive Oxygen Species In vivo.- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin.- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes.- Examining Protein Stability and Its Relevance for Plant Growth and Development.- Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics.- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells.- Fluorescence Cross-Correlation Spectroscopy of Plant Proteins.- The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS).- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways.- Phosphatase Activities Analyzed by in vivo Expressions.- Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences.- Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins.- Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay.- Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation.- RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.

From the reviews:

"This text pertinently addresses the field of signal transduction in plants. ... This volume covers a wide range of basic and cutting edge techniques that can be used in any number of areas of plant signaling research. ... This volume ... covers a wide range of cutting edge techniques that are leading to novel insight into signal transduction pathways in plant biology. ... this is a fine reference text that surveys a range of current plant experimental tools and techniques." (Beronda L. Montgomery, Plant Science Bulletin, October, 2009)