Plant Proteomics

Total Protein Extraction with TCA-Acetone.- Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues.- Protein Extraction from Cereal Seeds.- Protein Extraction from Xylem and Phloem Sap.- Protein Extraction from Woody Plants.- Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis.- Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis.- Extraction of Nuclear Proteins from Root Meristematic Cells.- Extraction of Nuclear Proteins.- Isolation of Cell Wall Proteins from Medicago sativa Stems.- Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis.- Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis.- Two-Dimensional Electrophoresis for Plant Proteomics.- Visible and Fluorescent Staining of Two-Dimensional Gels.- Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum.- Quantitative Analysis of 2D Gels.- Multivariate Data Analysis of Proteome Data.- Edman Sequencing of Proteins from 2D Gels.- Peptide Mass Fingerprinting.- Protein Identification Using Nano Liquid Chromatography-Tandem Mass Spectrometry.- Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots.- Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS.- The PROTICdb Database for 2-DE Proteomics.- Identification of Phosphorylated Proteins.- Plant Proteomics and Glycosylation.- Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants.- Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis.- Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions.- Phosphorylation Studies Using Plant Protein Microarrays.