Plant Innate Immunity
Methods and Protocols
(Sprache: Englisch)
The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating...
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The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.
Inhaltsverzeichnis zu „Plant Innate Immunity “
Preface...Table of Contents...Contributing Authors... 1 Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains Jay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, and Kee Hoon Sohn 2 Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine- Acetylated Proteins Expressed in planta Maxime Escouboué and Laurent Deslandes 3 Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA Karl J. Schreiber and Jennifer D. Lewis 4 A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions Mustafa Adhab and James E. Schoelz 5 Quantification of Extracellular ATP in Plant Suspension Cell Cultures Sowmya R. Ramachandran, Sonika Kumar, and Kiwamu Tanaka 6 Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions Gerit Bethke and Jane Glazebrook 7 Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, and Pallob Kundu 8 Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET- FLIM Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, and Laurent Deslandes 9 Analysis of DNA Methylation Profile in Plants by Chop-PCRPratiti Dasgupta and Shubho Chaudhuri 10 Change in Nucleosome Dynamics during Stress Responses in Plants Amit Paul and Shubho Chaudhuri 11 Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry Leiyun Yang, Zhixue Wang, and Jian Hua 12 A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, and Emmanuel Liscum 13 Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant
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Pathogen Pseudomonas syringae Conner J. Rogan and Jeffrey C. Anderson 14 Quantification of Cauline Leaf Abscission in Response to Plant Pathogens O. Rahul Patharkar 15 Methods for Replicating Leaf Vibrations Induced by Insect Herbivores Sabrina C.J. Michael, Heidi A. Appel, and Reginald B. Cocroft 16 Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes Marieke van Hulten, Sayantani Chatterjee, and Harrold A. van den Burg 17 The Apple Fruitlet Model System for Fire Blight Disease Sara M. Klee, Judith P. Sinn, and Timothy W. McNellis 18 Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins Sharon Pike, Walter Gassmann, and Jianbin Su 19 Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems Ankita Shrestha, Ahamed Khan, and Nrisingha Dey 20 Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants Ahamed Khan, Ankita Shrestha, and Nrisingha Dey 21 Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth Robert J. Kremer 22 A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance Eduardo Balsanelli, Vânia Carla Pankievicz, Valter Antonio Baura, Fábio de Oliveira Pedrosa, and Emanuel Maltempi de Souza Subject Index List...
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Bibliographische Angaben
- 2019, 1st ed. 2019, XIII, 260 Seiten, 42 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Walter Gassmann
- Verlag: Springer, Berlin
- ISBN-10: 1493994573
- ISBN-13: 9781493994571
- Erscheinungsdatum: 30.04.2019
Sprache:
Englisch
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