Nonribosomal Peptide and Polyketide Biosynthesis

Structural Biology of Nonribosomal Peptide Synthetases.- The Assembly Line Enzymology of Polyketide Biosynthesis.- Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay.- Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains.- Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases.- Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis.- Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro.- The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis.- Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC-MS/MS-Based Proteomics.- Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi.- In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.- Secondary Metabolic Pathway-Targeted Metabolomics.- Annotating and Interpreting Linear and CyclicPeptide Tandem Mass Spectra.- Bioinformatics Tools for the Discovery of NewNonribosomal Peptides.- The Use of ClusterMine360 for the Analysis ofPolyketide and Nonribosomal Peptide Biosynthetic Pathways.- Alignment-FreeMethods for the Detection and Specificity Prediction of Adenylation Domains.- Characterizationof Nonribosomal Peptide Synthetases with NRPSsp.