Neural Stem Cells

Definitions and Criteria for Stem Cells.- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard.- Isolation and Culture of NSCs.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb.- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- In Vitro Studies: A. Cellular Techniques.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.- Telomere Neurobiology.- Chromosomal Mosaicism in Neural Stem Cells.- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype.- In Vitro Migration Assays of Neural Stem Cells.- Measuring Apoptosis in Neural Stem Cells.- Markers of Adult Neural Stem Cells.- In Vitro Studies: B. Molecular Techniques.- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR.- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells.- Electroporation Strategies for Genetic Manipulation and Cell Labeling.- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches.- Studies for Transplantation.- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo.- Immunocytochemical Analysis of Neuronal Differentiation.- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold.- Labeling Stem Cells In

From the reviews of the second edition:



"Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. ... The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met. Neurologists, neurosurgeons, and neurosciences are the main audience. ... This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries." (Celso Agner, Doody's Review Service, June, 2008)

"Neural Stem Cells: Methods and Protocols, Second Edition, provides a comprehensive overview on cutting-edge methods for scientists working on questions related to neural stem-cell biology. ... The volume outlines methods and protocols that allow investigating mechanisms, which regulate self-renewal, cell survival and differentiation of NSCs. A better understanding of these processes is of great importance for future successful applications of NSCs in neurodegenerative diseases and injuries." (S. Atanasoski, Schweizer Archiv für Neurologie und Psychiatrie, April, 2009)