Molecular Bacteriology: Protocols and Clinical Applications

Impact of Molecular Methods on Clinical Bacteriology.- Genomic DNA Digestion and Ribotyping.- Pulsed-Field Gel Electrophoresis.- Plasmid Analysis.- DNA Amplification.- Arbitrarily Primed PCR Methods for Studying Bacterial Diseases.- Genomic Fingerprinting by Application of rep-PCR.- Molecular Approaches to the Identification of Streptococci.- Pneumococcal Diseases.- Molecular Approaches in Mycobacterium tuberculosisand Other Infections Caused by Mycobacterium Species.- Diagnosis and Epidemiology of Diphtheria.- Diagnosis and Epidemiology of Infections Caused by Legionella spp..- Molecular Methods for Haemophilus influenzae.- The Impact o Molecular Techniques on the Study of Meningococcal Disease.- Gonorrhea.- Chancroid.- Mycoplasma and Ureaplasma Infections.- Application of Molecular Methods to the Study of Infections Caused by Salmonella spp..- Cholera.- Diagnosisand Investigation of Diarrheagenic Escherichia coli.- Campylobacter Infections.- Detection and Typing of Helicobacter pylori.- Nosocomial Infections Caused by Staphylococci.- Application of Molecular Techniques to the Study of Nosocomial Infections Caused by Enterococci.- Molecular Approaches for the Detection and Identification of ?-Lactamases.- Biochemical and Enzyme Kinetic Applications for the Characterization of ?Lactamases.- ?Lactam Resistance Mediated by Changes in Penicillin-Binding Proteins.- The Application of Molecular Techniques for the Study of Aminoglycoside Resistance.- Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria.- Quinolone Resistance.- Resistance to Tetracyclines, Macrolides, Trimethoprim and Sulfonamides.

"The book is authoritative, as its many contributors are all experts in their fields; their contributions have been well coordinated by the editors, resulting in a unified work. . .The book is sturdily bound in hardback to withstand the considerable use it deserves and will undoubtedly get. It represents very good value and I recommend that all laboratories interested in molecular diagnosis in bacteriology should buy a copy of it."-Journal of Medical Microbiology

"Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories...Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations....This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases."-Doody's Health Science Book Review Journal

". . .a book that will be useful not only to the experienced researchers, but also to the newcomer."-Laboratoriums Medizin