Mixed-Mode Chromatography
Principles, Methods, and Applications
(Sprache: Englisch)
The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to...
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Klappentext zu „Mixed-Mode Chromatography “
The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.
Inhaltsverzeichnis zu „Mixed-Mode Chromatography “
Chapter 1.Historical Perspectives.- Chapter 2.Theoretical Background.- Chapter 3.Modes of Chromatographic Separation.- Chapter 4.Chromatographic Matrices .- Chapter 5.Ligand Design.- Chapter 6.Retention Mechanisms.- Chapter 7.Stationary Phases.- Chapter 8.Mobile Phases and Elution.- Chapter 9.Method Development.- Chapter 10.Applications in Biopharmaceutical Processing. .
Autoren-Porträt von Qian-Hong Wan
Qian-Hong Wan is a professor in the Pharmaceutical Analysis department, School of Pharmaceutical Science and Technology, Tianjin University. He obtained his Ph.D. from Edinburgh University in 1993 and completed his two post-doctoral training at University of Amsterdam and Nottingham University. His research is focused on modern separation and analysis technology. Bibliographische Angaben
- Autor: Qian-Hong Wan
- 2022, 1st ed. 2021, XIII, 482 Seiten, 65 farbige Abbildungen, Masse: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Verlag: Springer, Berlin
- ISBN-10: 9811654875
- ISBN-13: 9789811654879
Sprache:
Englisch
Pressezitat
"The present book represents a key reference in the field of mixed-mode chromatography ... . The ten chapters are well balanced, with many fundamental concepts on retention mechanisms as well as more applicative ones, which give the reader a complete overview of the state-of-the-art and future perspectives in this emerging, yet very promising field. Latest innovations related to fabrication of mixed-mode materials are also covered, with particular attention to computer-aided approaches to optimise their chemical structure and properties." (Alberto Cavazzini, Analytical and Bioanalytical Chemistry, Vol. 415, 2023)
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